Kameda Y
Department of Anatomy, Fukuoka University School of Medicine, Japan.
Am J Anat. 1990 Jun;188(2):185-98. doi: 10.1002/aja.1001880208.
Colloid-containing follicles and ciliated cysts in the hypophysial pars tuberalis of guinea pigs at various ages ranging from 5 days to 36 months were examined by periodic acid-Schiff (PAS) reaction, immunohistochemistry, and electron microscopy. The follicles storing PAS-positive colloid were encountered in the pars tuberalis of all guinea pigs examined, although only a few were present in young animals. The follicles gradually increased in number with age. The largest number of follicles was found in the senile male group: 141.3 +/- 11.9, about 10 times the number in the 5-day-old male group. The follicles were scattered throughout the entire length of the pars tuberalis. Follicles with enlarged luminal cavities were concentrated in the ventral caudal region surrounding the infundibular stem and merges with the pars distalis. Three different types of follicles were found by electron microscopy: 1) those surrounded by nongranulated follicular cells that may correspond to the stellate-follicular cells in the pars distalis, 2) those surrounded by specific cells that were packed with vesicular inclusions, and 3) those surrounded by granulated cells that may be gonadotropes. In the follicles lined by non-granulated follicular cells, long, prominent microvilli and cytoplasmic processes protruding into the lumen and invaginations of colloid were often observed at the apical cell region. The follicles lined by the specific cells having numerous vesicles were localized only in the ventral caudal portion. The vesicles ranged from 200 to 700 nm in diameter, and the outer surface of their limiting membrane was partly studded with ribosomes. Gonadotropes immunoreactive to the luteinizing hormone (LH) and follicle-stimulating hormone (FSH) antisera were distributed in the guinea pig pars tuberalis. As well as the typical follicles described above, the follicles composed solely of granulated cells showed microvilli protruding into the cavities and junctional complexes at the apical lateral surface. They stored heterogeneous materials in the lumina. Some secretory granules gave the appearance of being discharged into the lumen. Ciliated cysts were frequently observed in the pars tuberalis; their incidence was 71.7%. The ciliated cysts were much larger than colloid-containing follicles. Cystic cavities were only partly filled with heterogeneous materials showing colloid-like, flocculent, and granular features.
采用过碘酸希夫（PAS）反应、免疫组织化学和电子显微镜技术，对5日龄至36月龄不同年龄豚鼠垂体结节部含胶体的滤泡和纤毛囊肿进行了检查。在所检查的所有豚鼠的垂体结节部均发现了储存PAS阳性胶体的滤泡，不过幼龄动物中只有少数滤泡。滤泡数量随年龄逐渐增加。老年雄性组的滤泡数量最多：141.3±11.9个，约为5日龄雄性组数量的10倍。滤泡散布于垂体结节部的全长。管腔扩大的滤泡集中在围绕漏斗柄的尾腹侧区域，并与远侧部相连。通过电子显微镜发现了三种不同类型的滤泡：1）被无颗粒滤泡细胞包围的滤泡，可能对应于远侧部的星状滤泡细胞；2）被充满囊泡内含物的特定细胞包围的滤泡；3）被可能是促性腺激素细胞的颗粒细胞包围的滤泡。在由无颗粒滤泡细胞内衬的滤泡中，常在顶端细胞区域观察到长而突出的微绒毛和伸入管腔的细胞质突起以及胶体的内陷。由具有大量囊泡的特定细胞内衬的滤泡仅位于尾腹侧部分。囊泡直径在200至700纳米之间，其限制膜的外表面部分布满核糖体。对促黄体生成素（LH）和促卵泡激素（FSH）抗血清呈免疫反应的促性腺激素细胞分布于豚鼠垂体结节部。除了上述典型滤泡外，仅由颗粒细胞组成的滤泡在腔内向内突出微绒毛，并在顶端侧面有连接复合体。它们在管腔内储存异质物质。一些分泌颗粒看起来正排入管腔。在垂体结节部经常观察到纤毛囊肿；其发生率为71.7%。纤毛囊肿比含胶体的滤泡大得多。囊腔仅部分充满具有胶体样、絮状和颗粒状特征的异质物质。
