Kameda Y
Anat Rec. 1986 Oct;216(2):175-80. doi: 10.1002/ar.1092160209.
The effect of aging on the formation of C cell follicles was examined in the thyroid gland of guinea pigs at various ages ranging from 1 to 29 months. The C cell follicles were demonstrated with the immunoperoxidase methods by using anticalcitonin and antisomatostatin antisera and with PAS reaction. They were already detected in 1-month-old guinea pigs but in low number. Thyroid glands from 1- to 14-month-old animals contained only a small number of C cell follicles and did not reveal the age-related increase. In aged guinea pigs (20- to 29-month-old), a dramatic increase of C cell follicles was found, about 13 times as high as the number of other age groups. The C cell follicles through all age groups were present in large clusters of C cells. In the aged guinea pigs, nodular large aggregates of C cells regarded as C cell hyperplasia occurred and numerous C cell follicles were formed in the large cell aggregates. Thus, the conspicuous increase of C cell follicles in aged animals was associated with a proliferative abnormality of C cells. The C cells forming follicles showed moderate to weak immunoreactivity for calcitonin, whereas they showed very intense immunoreactivity for somatostatin. In addition, the colloidlike and flocculent materials stored in the follicular lumina, which were consistently PAS-positive, were weakly immunoreactive to somatostatin but nonreactive to calcitonin.
在1至29个月龄的豚鼠甲状腺中，研究了衰老对C细胞滤泡形成的影响。采用抗降钙素和抗生长抑素抗血清，通过免疫过氧化物酶法及PAS反应显示C细胞滤泡。在1月龄的豚鼠中已检测到C细胞滤泡，但数量较少。1至14月龄动物的甲状腺仅含有少量C细胞滤泡，未显示出与年龄相关的增加。在老年豚鼠（20至29月龄）中，发现C细胞滤泡显著增加，约为其他年龄组数量的13倍。所有年龄组的C细胞滤泡均以大的C细胞簇形式存在。在老年豚鼠中，出现了被视为C细胞增生的结节状大的C细胞聚集体，并且在大的细胞聚集体中形成了许多C细胞滤泡。因此，老年动物中C细胞滤泡的显著增加与C细胞的增殖异常有关。形成滤泡的C细胞对降钙素显示中度至弱免疫反应性，而对生长抑素显示非常强的免疫反应性。此外，滤泡腔内储存的胶体样和絮状物质始终呈PAS阳性，对生长抑素呈弱免疫反应性，对降钙素无反应。
