Zhang Hai-Feng, Zhang Bo, Zhang Xiao-Dong, Gao Feng
Department of Physiology, Fourth Military Medical University, Xi'an 710032, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2004 Nov;20(4):410-4.
Single cardiomyocytes were isolated from adult rat hearts and myocyte contraction was recorded by a video-based motion edge-detection system.
Single ventricular myocytes were enzymatically isolated, loaded with fura-2/AM (0.5 micromol/L) for 30 min in dark at room temperature and field-stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video-based motion edge-detection system.
Using the video-based motion edge-detection system, one may real-time beat-to-beat observe and record the amplitude, duration and velocity of both myocyte shortening/ relengthening and intracellular calcium transient, and then directly describe the change of isolated ventricular myocyte mechanics.
Direct measurement of isolated ventricular myocyte mechanics using the video-based motion edge-detection system is an increasingly important technique in cardiac physiology that provides fundamental information on myogenic and excitation-contraction coupling of the heart in drug intervention and gene manipulation.
从成年大鼠心脏中分离单个心肌细胞，并通过基于视频的运动边缘检测系统记录心肌细胞收缩情况。
酶法分离单个心室肌细胞，在室温黑暗条件下用fura-2/AM（0.5微摩尔/升）加载30分钟，然后进行场刺激（0.5赫兹，5毫秒），通过基于视频的运动边缘检测系统同时评估心肌细胞收缩和细胞内荧光强度。
使用基于视频的运动边缘检测系统，可以逐搏实时观察和记录心肌细胞缩短/再延长的幅度、持续时间和速度以及细胞内钙瞬变，进而直接描述分离的心室肌细胞力学变化。
使用基于视频的运动边缘检测系统直接测量分离的心室肌细胞力学是心脏生理学中一项日益重要的技术，它为药物干预和基因操作中关于心脏的肌源性和兴奋-收缩偶联提供了基础信息。
