Ogino K, Eda-Fujiwara H, Fujiwara H, Ishikawa H
Zoological Institute, Faculty of Science, University of Tokyo, Japan.
J Mol Evol. 1990 Jun;30(6):509-13. doi: 10.1007/BF02101106.
In order to determine why the aphid 28S rRNA lacks the hidden break otherwise found in insects, the structure of the region of the aphid ribosomal DNA (rDNA) corresponding to the gap region, which in other insect rDNA transcripts is excised posttranscriptionally, was studied. Sequence comparison suggested that, in contradistinction to what is found in rDNA transcripts of other insects, a stem-loop structure formed in this region of the aphid rDNA transcript is not AU-rich. Nor did the loop of the aphid molecule contain the UAAU tract that can be a signal for the introduction of the hidden break, suggesting that in this particular region the aphid 28S rRNA resembles 28S rRNAs of deuterostomes, which do not contain the hidden break.
为了确定蚜虫28S rRNA为何没有在其他昆虫中发现的隐藏断裂,研究了蚜虫核糖体DNA(rDNA)中与间隙区域相对应的区域的结构,该区域在其他昆虫rDNA转录本中是在转录后被切除的。序列比较表明,与其他昆虫的rDNA转录本不同,蚜虫rDNA转录本的这一区域形成的茎环结构并非富含AU。蚜虫分子的环中也没有可作为引入隐藏断裂信号的UAAU序列,这表明在这个特定区域,蚜虫28S rRNA类似于后口动物的28S rRNA,后者不含有隐藏断裂。