Fujiwara H, Ishikawa H
Nucleic Acids Res. 1986 Aug 26;14(16):6393-401. doi: 10.1093/nar/14.16.6393.
We determined the structure around the gap region between 28S alpha and 28S beta rRNA of Bombyx mori, a lepidopteran insect, to know the introduction mechanism of the hidden break, an interruption of the phosphodiester bond specific to the 28S rRNA of protostomes. Sequence analysis and S1 nuclease mapping suggested that a stretch of 30 nucleotides is excised from the mid region of the 28S rRNA to generate the hidden break. The length of excluded stretch was very various among three insects so far studied. However, the gap responsible for the hidden break was located in a fixed position in the 28S rRNA irrespective of insect species. It was suggested that extremely AU-rich sequence including the specific UAAU tract forming a loop can be a signal for the introduction of the hidden break. The same signal seemed also involved in splitting the dipteran 5.8S rRNA.
我们确定了鳞翅目昆虫家蚕28Sα和28Sβ核糖体RNA之间间隙区域的结构，以了解隐藏断裂的引入机制，隐藏断裂是原口动物28S核糖体RNA特有的磷酸二酯键中断。序列分析和S1核酸酶图谱表明，从28S核糖体RNA的中间区域切除一段30个核苷酸以产生隐藏断裂。到目前为止，在已研究的三种昆虫中，排除片段的长度差异很大。然而，无论昆虫种类如何，导致隐藏断裂的间隙都位于28S核糖体RNA的固定位置。有人提出，包括形成环的特定UAAU序列在内的富含AU的序列可能是引入隐藏断裂的信号。相同的信号似乎也参与了双翅目5.8S核糖体RNA的分裂。
