School of Pharmacy, Pharmacy Australia Centre of Excellence, The University of Queensland, 20 Cornwall Street, Woolloongabba, QLD 4102, Australia.
J Chromatogr A. 2011 Jan 14;1218(2):359-61. doi: 10.1016/j.chroma.2010.11.047. Epub 2010 Nov 27.
Matrix matching is used in analysis to compensate for matrix effects that influence analytical response. It has been a widely discussed topic in electro-spray mass spectrometry where the ionization suppression is a major problem in accurate quantitative analysis. However, the unique strength of mass spectrometry to detect and quantify accurately a co-eluting stable isotope labelled internal standard offers an easy solution to the ionization suppression problem. Given the fact that it is impossible to match the matrix of the calibration standards with all samples, mass spectrometry allows accurate quantitation without the need for matrix matching, as long as the internal standard co-elutes with the analyte of interest. If the analyte and internal standard co-elute, the slope of the calibration curve analyte response/internal standard vs. analyte concentration is independent of the matrix composition, eliminating the need for matrix matching.
基质匹配在分析中用于补偿影响分析响应的基质效应。在电喷雾质谱中，基质效应是准确定量分析的主要问题，因此它是一个广泛讨论的话题。然而，质谱具有准确检测和定量共洗脱稳定同位素标记内标的独特优势，为解决电离抑制问题提供了简单的解决方案。鉴于校准标准品的基质不可能与所有样品完全匹配的事实，只要内标与感兴趣的分析物共洗脱，质谱就可以在无需基质匹配的情况下进行准确的定量，因为分析物和内标共洗脱时，校准曲线分析物响应/内标与分析物浓度的斜率与基质组成无关，因此无需进行基质匹配。
