4-羟基苯甲醚对体内黑色素瘤细胞的非选择性作用。

Non-selective action of 4-hydroxyanisole on melanoma cells in vivo.

作者信息

Schwabe K, Fichtner I, Tschiersch B

机构信息

Central Institute for Cancer Research, Academy of Sciences of G.D.R., Berlin-Buch.

出版信息

Pigment Cell Res. 1990 Jan-Feb;3(1):8-10. doi: 10.1111/j.1600-0749.1990.tb00255.x.

DOI:10.1111/j.1600-0749.1990.tb00255.x

PMID:2116000

Abstract

In order to clarify the role of tyrosinase (E.C. 1.14.18.1) in the cytotoxicity of 4-hydroxyanisole (4HA) in vivo, we have compared the therapeutic effects of 4HA on the B16 melanoma and Harding-Passey melanoma, which differ significantly in their tyrosinase content. The observed therapeutic effects are moderate and similar in both tumors. Therefore, there is no evidence for an increase of the cytotoxic effect of 4HA by tyrosinase in vivo. Application of 4HA to mice carrying B16 melanoma and Harding-Passey melanoma results in an inhibition of [3H]-TdR incorporation into melanoma DNA as well as into DNA of liver, intestine, kidney, and spleen. There is no selective activity on melanoma cells by 4HA in vivo. Therefore, in the therapy of human melanoma by 4HA, side effects on normal tissues cannot be excluded.

摘要

为了阐明酪氨酸酶（E.C. 1.14.18.1）在4-羟基茴香醚（4HA）体内细胞毒性中的作用，我们比较了4HA对B16黑色素瘤和哈丁-帕西黑色素瘤的治疗效果，这两种黑色素瘤的酪氨酸酶含量差异显著。观察到的治疗效果在两种肿瘤中均为中等且相似。因此，没有证据表明酪氨酸酶在体内会增强4HA的细胞毒性作用。将4HA应用于携带B16黑色素瘤和哈丁-帕西黑色素瘤的小鼠，会抑制[3H]-胸腺嘧啶核苷掺入黑色素瘤DNA以及肝脏、肠道、肾脏和脾脏的DNA中。4HA在体内对黑色素瘤细胞没有选择性活性。因此，在4HA治疗人类黑色素瘤时，不能排除对正常组织产生副作用。