多胺对酪氨酸酶活性的影响。
The effect of polyamines on tyrosinase activity.
作者信息
Galindo J D, Martinez J H, Lopez-Ballester J A, Peñafiel R, Solano F, Lozano J A
机构信息
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Murcia, Spain.
出版信息
Biochem Int. 1987 Dec;15(6):1151-8.
The effect of several polyamines on the activity of tyrosinase from different sources has been studied. Diaminoethane, 1,3-diaminopropane and putrescine activated tyrosinase from Harding-Passey mouse melanoma but did not activate frog epidermis or mushroom tyrosinases. 1,3-diaminopropane was the strongest activator (Ka = 0.23 mM). The activation was saturable and dependent on the ionic strength. Cadaverine, 1,6-diaminohexane and spermidine had no effect on any tyrosinase. However, spermine inhibited melanoma tyrosinase more than the mushroom and frog epidermis enzymes. These results show that the effect of polyamines on mammalian tyrosinase is due to direct enzyme-oligoamine interactions rather than to a nonspecific action on L-dopa oxidation products, and suggest that physiological polyamines might play a modulatory role on mammalian melanogenesis.
研究了几种多胺对不同来源酪氨酸酶活性的影响。二氨基乙烷、1,3 - 二氨基丙烷和腐胺可激活哈丁 - 帕西小鼠黑色素瘤中的酪氨酸酶，但不能激活青蛙表皮或蘑菇中的酪氨酸酶。1,3 - 二氨基丙烷是最强的激活剂（Ka = 0.23 mM）。这种激活是可饱和的，并且依赖于离子强度。尸胺、1,6 - 二氨基己烷和亚精胺对任何酪氨酸酶均无影响。然而，精胺对黑色素瘤酪氨酸酶的抑制作用比对蘑菇和青蛙表皮中的酶更强。这些结果表明，多胺对哺乳动物酪氨酸酶的作用是由于酶与寡胺的直接相互作用，而不是对L - 多巴氧化产物的非特异性作用，并表明生理性多胺可能在哺乳动物黑色素生成中起调节作用。
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