Sidira Marianthi, Galanis Alex, Ypsilantis Petros, Karapetsas Athanasios, Progaki Zoi, Simopoulos Constantinos, Kourkoutas Yiannis
Applied Microbiology and Molecular Biotechnology Research Group, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
J Mol Microbiol Biotechnol. 2010;19(4):224-30. doi: 10.1159/000321115. Epub 2010 Dec 14.
The aim of the present study was to assess the survival of free and immobilized Lactobacillus casei ATCC 393 on apple pieces, contained in probiotic-fermented milk, after gastrointestinal (GI) transit and to investigate the potential regulation of intestinal microbial flora in a rat model. In in vitro GI stress tolerance tests, immobilized L. casei ATCC 393 exhibited significantly higher survival rates compared to free cells. At a second stage, probiotic-fermented milk produced by either free or immobilized cells was administered orally at a single dose or daily for 9 days in Wistar rats. By 12 h after single-dose administration, both free and immobilized cells were detected by microbiological and molecular analysis at levels ≥6 logCFU/g of feces. Moreover, daily administration led to significant reduction of staphylococci, enterobacteria, coliforms and streptococci counts. In conclusion, L. casei ATCC 393 contained in fermented milk survived GI transit and modulated intestinal microbiota.
本研究的目的是评估益生菌发酵乳中游离和固定化的干酪乳杆菌ATCC 393在苹果片上经胃肠道转运后的存活率，并在大鼠模型中研究其对肠道微生物群的潜在调节作用。在体外胃肠道应激耐受性试验中，与游离细胞相比，固定化的干酪乳杆菌ATCC 393表现出显著更高的存活率。在第二阶段，将游离或固定化细胞生产的益生菌发酵乳以单剂量或每日一次的方式口服给予Wistar大鼠，持续9天。单剂量给药后12小时，通过微生物学和分子分析检测到游离和固定化细胞在粪便中的含量均≥6 logCFU/g。此外，每日给药导致葡萄球菌、肠杆菌、大肠菌群和链球菌数量显著减少。总之，发酵乳中含有的干酪乳杆菌ATCC 393在胃肠道转运后存活，并调节了肠道微生物群。
