Applied Microbiology and Molecular Biotechnology Research Group, Department of Molecular Biology and Genetics, Democritus University of Thrace, Dragana, Alexandroupolis, Greece.
Anaerobe. 2012 Aug;18(4):417-20. doi: 10.1016/j.anaerobe.2012.04.002. Epub 2012 Apr 17.
Adhesion to the intestine represents a critical parameter for probiotic action. In this study, the adhesion ability of Lactobacillus casei ATCC 393 to the gastrointestinal tract of Wistar rats was examined after single and daily administration of fermented milk containing either free or immobilized cells on apple pieces. The adhesion of the probiotic cells at the large intestine (cecum and colon) was recorded at levels ≥6 logCFU/g (suggested minimum levels for conferring a probiotic effect) following daily administration for 7 days by combining microbiological and strain-specific multiplex PCR analysis. Single dose administration resulted in slightly reduced counts (5 logCFU/g), while they were lower at the small intestine (duodenum, jejunum, ileum) (≤3 logCFU/g), indicating that adhesion was a targeted process. Of note, the levels of L. casei ATCC 393 were enhanced in the cecal and colon fluids both at single and daily administration of immobilized cells (6 and 7 logCFU/g, respectively). The adhesion of the GI tract was transient and thus daily consumption of probiotic products containing the specific strain is suggested as an important prerequisite for retaining its levels at an effective concentration.
黏附到肠道是益生菌发挥作用的一个关键参数。在这项研究中，研究了经口给予含有游离或固定在苹果片上的细胞的发酵乳后，Lactobacillus casei ATCC 393 对 Wistar 大鼠胃肠道的黏附能力。通过微生物学和种特异性多重 PCR 分析相结合，在连续 7 天每日给予后，在大肠（盲肠和结肠）中记录到益生菌细胞的黏附水平≥6 logCFU/g（被认为是发挥益生菌作用的最小水平）。单次给予后，计数略有减少（5 logCFU/g），而在小肠（十二指肠、空肠、回肠）中则更低（≤3 logCFU/g），表明黏附是一个靶向过程。值得注意的是，在单次和每日给予固定细胞后，L. casei ATCC 393 在盲肠和结肠液中的水平均得到增强（分别为 6 和 7 logCFU/g）。肠道黏附是短暂的，因此建议每日食用含有特定菌株的益生菌产品，这是保持其有效浓度的重要前提。
