Grigorian A S, Kruglyakov P V, Taminkina U A, Efimova O A, Pendina A A, Voskresenskaya A V, Kuznetsova T V, Polyntsev D G
Trans-Technology Ltd, St. Petersburg, Russia.
Bull Exp Biol Med. 2010 Dec;150(1):125-30. doi: 10.1007/s10517-010-1086-x.
Transplantation of human bone marrow mesenchymal stem cells is considered as a promising therapeutic approach to the therapy of many diseases. However, the problem of possible alterations of the properties of mesenchymal stem cells during their expansion in in vitro cultures before transplantation is not solved. In our study, one of two hundred examined cultures of mesenchymal stem cell cultures derived from donors without bone marrow pathologies and developed under standard culturing conditions demonstrated spontaneous disturbances in morphology, proliferation, and karyotype at early passages. The cells of this abnormal culture retained immunophenotype characteristic of normal mesenchymal stem cells, but some of them (15-25%) had numerous numerical and structural chromosome aberrations.
人类骨髓间充质干细胞移植被认为是治疗多种疾病的一种有前景的治疗方法。然而，间充质干细胞在移植前体外培养扩增过程中其特性可能发生改变的问题尚未得到解决。在我们的研究中，在标准培养条件下从无骨髓病变的供体来源的两百份间充质干细胞培养物中，有一份在早期传代时出现了形态、增殖和核型的自发异常。这种异常培养物中的细胞保留了正常间充质干细胞的免疫表型，但其中一些细胞（15%-25%）有大量的染色体数目和结构异常。
