Nikitina V A, Osipova E Yu, Katosova L D, Rumyantsev S A, Skorobogatova E V, Shamanskaya T V, Bochkov N P
Medical Genetic Research Center,Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2011 Mar;150(5):627-31. doi: 10.1007/s10517-011-1207-1.
Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.
研究了骨髓多能间充质基质细胞的免疫表型、增殖率和遗传稳定性参数。尽管传代至11-12代时增殖活性降低，但细胞仍保留了特征性免疫表型。评估了6号、8号、11号常染色体和性染色体自发非整倍体的发生率。检测到两株携带非整倍体细胞克隆的间充质基质细胞培养物：一株为8号染色体三体，另一株为X染色体单体。结果表明，在体外培养过程中，间充质基质细胞可能发生遗传转化并出现核型异常的细胞。
