Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600007, India.
Parasitol Res. 2011 Apr;108(4):1059-62. doi: 10.1007/s00436-010-2182-6. Epub 2010 Dec 14.
As a first attempt to generate sequence information from the protein-coding genes of the genomically unknown parasite, Eimeria brunetti, a cDNA library was generated from purified sporozoites in the λTriplEx2™ vector. Analysis of 283 expressed sequence tags (ESTs) from the cDNA library constructed revealed 12 contigs (26 ESTs) and 257 singletons. BLASTx analysis revealed that 50 transcripts had significant matches to known proteins, whereas the remaining 233 had no significant matches, probably representing novel genes. Based on Gene Ontology classification, the transcripts were categorized as biological process (46 ESTs), molecular function (37 ESTs), and cellular component (19 ESTs). The transcripts analyzed show maximum homology to the apicomplexan parasite Toxoplasma gondii. Despite the small number of transcripts, this is the first transcriptome analysis of E. brunetti and provides preliminary data that will increase understanding of parasite biological function.
作为首次尝试从基因组未知的寄生虫艾美耳球虫的蛋白质编码基因中生成序列信息，我们从纯化的孢子中生成了一个 cDNA 文库在 λTriplEx2™载体。对 cDNA 文库构建的 283 个表达序列标签 (EST) 的分析显示有 12 个重叠群 (26 个 EST) 和 257 个单 EST。BLASTx 分析表明，50 个转录本与已知蛋白有显著匹配，而其余 233 个没有显著匹配，可能代表新基因。基于基因本体论分类，这些转录本被归类为生物过程 (46 个 EST)、分子功能 (37 个 EST) 和细胞成分 (19 个 EST)。分析的转录本与顶复门寄生虫刚地弓形虫的同源性最高。尽管转录本数量较少，但这是艾美耳球虫的首次转录组分析，提供了将增加对寄生虫生物学功能理解的初步数据。
