Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46 East Construction Road, Xinxiang, Henan, China.
Biochem Genet. 2011 Apr;49(3-4):161-76. doi: 10.1007/s10528-010-9396-2. Epub 2010 Dec 15.
To investigate the function and evolutionary origin of the SOXE subgroup, we amplified the genomic DNA of Misgurnus anguillicaudatus using a pair of degenerate primers. Using RACE, we obtained two versions of Sox8 (MaSox8a and MaSox8b) from M. anguillicaudatus. The overall sequence identity of the deduced amino acids from the two genes was 54.38%, with only one amino acid change in the high-mobility group domain. Southern blotting and evidence from the phylogenetic tree provided further proof for the existence of two Sox8 genes at the genomic level. This is the first evidence of two distinct Sox8 genes in Cypriniformes. Semi-quantitative and real-time quantitative PCR assays showed the expression trend of the genes was opposite in early embryonic development, and both were expressed ubiquitously in several adult tissues. The similar expression patterns indicated that MaSox8a and MaSox8b have possible overlapping functions.
为了研究 SOXE 亚组的功能和进化起源，我们使用一对简并引物扩增了泥鳅基因组 DNA。通过 RACE，我们从泥鳅中获得了两种版本的 Sox8（MaSox8a 和 MaSox8b）。这两个基因推导的氨基酸的整体序列同一性为 54.38%，只有在高迁移率组结构域中有一个氨基酸的变化。Southern 印迹和系统发育树的证据进一步证明了 Sox8 基因在基因组水平上的存在。这是首次在鲤形目中发现两个不同的 Sox8 基因。半定量和实时定量 PCR 分析表明，这些基因在早期胚胎发育过程中的表达趋势相反，在几种成年组织中均广泛表达。相似的表达模式表明 MaSox8a 和 MaSox8b 可能具有重叠的功能。
