Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46# East of Construction Road, Xinxiang, 453007, Henan, People's Republic of China.
Fish Physiol Biochem. 2013 Oct;39(5):1341-51. doi: 10.1007/s10695-013-9788-4. Epub 2013 Mar 28.
A number of genetic studies have established that Sox10 is a transcription factor associated with neurogenesis in vertebrates. We have isolated a homologue of Sox10 gene from the brain of Misgurnus anguillicaudatus by using homologous cloning and RACE method, designated as MaSox10b. The full-length cDNA of MaSox10b contained a 311 bp 5'UTR, a 312 bp 3'UTR and an ORF encoding a putative protein of 490 amino acids with a characteristic HMG-box DNA-binding domain of 79 amino acids (aa: 105-183). Phylogenetic tree shows that the MaSOX10b fits within the Sox10 clade and clusters firmly into Sox10b branches. During embryogenesis, MaSox10b was first detected in gastrulae stage. From somitogenesis stage and thereafter, distinct expression was observed in the medial neural tube, extending from the hindbrain through the posterior trunk. Taken together, these preliminary findings suggested that MaSox10b is highly conserved during vertebrate evolution and involved in a wide range of developmental processes including embryogenesis and neurogenesis.
一些遗传研究已经证实 Sox10 是一种与脊椎动物神经发生相关的转录因子。我们通过同源克隆和 RACE 方法从泥鳅脑中分离出 Sox10 基因的同源物，命名为 MaSox10b。MaSox10b 的全长 cDNA 包含一个 311bp 的 5'UTR、一个 312bp 的 3'UTR 和一个编码 490 个氨基酸的开放阅读框，其中包含一个具有 79 个氨基酸（aa：105-183）特征性 HMG 盒 DNA 结合域的假定蛋白。系统发育树表明，MaSOX10b 位于 Sox10 分支内，并且牢固地聚类到 Sox10b 分支中。在胚胎发生过程中，MaSox10b 首先在原肠胚期检测到。从体节形成阶段开始，在从中脑延伸到后躯的中侧神经管中观察到明显的表达。综上所述，这些初步发现表明，MaSox10b 在脊椎动物进化过程中高度保守，参与广泛的发育过程，包括胚胎发生和神经发生。
