Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, China.
Arch Virol. 2011 Apr;156(4):685-8. doi: 10.1007/s00705-010-0871-1. Epub 2010 Dec 16.
In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.
在之前的研究中，我们在小鼠中进行了猪乙型脑炎病毒的连续脑脑传递，并对 F5 和 F20 传代的适应鼠的变体进行了全基因组测序。在本研究中，我们分析了它们基因组序列之间的差异，发现非结构蛋白中有 12 个氨基酸取代。我们还评估了这两种变体在体外和体内哺乳动物细胞中的生长特性。我们的研究表明，与 F5 变体相比，F20 变体具有增强的生长特性和改变的毒力。因此，我们得出结论，猪乙型脑炎病毒非结构蛋白中的多个氨基酸取代改变了其在小鼠中的毒力。
