Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
Photosynth Res. 2011 Feb;107(2):133-8. doi: 10.1007/s11120-010-9603-3. Epub 2010 Dec 15.
Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of the synthetic monomeric BChl-f as well as its visible absorption and fluorescence emission spectra in a solution were identified and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Based on the above data, BChl-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e.
尚未在天然光养生物中发现的细菌叶绿素（BChl）-f 是通过化学修饰叶绿素-b 制备的。合成单体 BChl-f 的反相高效液相色谱保留时间以及其在溶液中的可见吸收和荧光发射光谱与从绿硫细菌的主要光捕获天线系统获得的其他天然存在的叶绿素色素 BChls-c/d/e 进行了鉴定和比较。基于上述数据，在产生 BChl-e 的三种绿色光合细菌中，BChl-f 的含量低于检测水平。
