Rendell N B, Taylor G W, Somerville M, Todd H, Wilson R, Cole P J
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, U.K.
Biochim Biophys Acta. 1990 Jul 16;1045(2):189-93. doi: 10.1016/0005-2760(90)90150-v.
The Gram negative organism, Pseudomonas aeruginosa, is often found in the lungs of patients with cystic fibrosis and other forms of severe bronchiectasis, where it secretes a number of extracellular toxins including the mono- and dirhamnolipids. The principal monorhamnolipid from P. aeruginosa has previously been identified as rhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoate (Rh-C10.C10). A number of related mono- and dirhamnolipids have been purified from cultures of a clinical isolate of P. aeruginosa and identified by fast atom bombardment and electron impact mass spectrometry: these contain the 3-hydroxyoctanoyl-3-hydroxydecanoate (C8.C10) and 3-hydroxydecanoyl-3-hydroxydodecanoate (C10.C12) homologues. Structural isomers were also present where the order of the lipid linkage was transposed (Rh-C10.C8 and Rh-C12.C10). Unsaturated mono- and dirhamnolipids containing the 3-hydroxydecanoyl-3-hydroxydodec-5-enoate (C10.C12:1) lipid were also present.
革兰氏阴性菌铜绿假单胞菌常存在于囊性纤维化患者及其他严重支气管扩张症患者的肺部，它会分泌多种细胞外毒素，包括单鼠李糖脂和双鼠李糖脂。此前已鉴定出铜绿假单胞菌产生的主要单鼠李糖脂为鼠李糖基-3-羟基癸酰基-3-羟基癸酸酯（Rh-C10.C10）。从一株铜绿假单胞菌临床分离株的培养物中纯化出了多种相关的单鼠李糖脂和双鼠李糖脂，并通过快原子轰击和电子轰击质谱进行了鉴定：这些物质含有3-羟基辛酰基-3-羟基癸酸酯（C8.C10）和3-羟基癸酰基-3-羟基十二酸酯（C10.C12）同系物。还存在脂质连接顺序颠倒的结构异构体（Rh-C10.C8和Rh-C12.C10）。此外，还存在含有3-羟基癸酰基-3-羟基-5-十二碳烯酸酯（C10.C12:1）脂质的不饱和单鼠李糖脂和双鼠李糖脂。
