高效液相色谱-荧光检测法测定血浆同型半胱氨酸水平
[Determination of homocysteine level in plasma by high performance liquids chromatography with fluorescence detection].
作者信息
Da Xu, Qian Ling-Jia
机构信息
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Aug;21(3):353-5.
AIM
To establish a method for detection of plasma total homocysteine with HPLC.
METHODS
The chromatography analysis was carried out using a Symmetry Shield RP18. The mobile phase was sodium acetate (0.08 mol/L) and methanol (1%) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F).
RESULTS
The average recoveries were 95.8 - 100.8% and the relative standard deviations were 1.2-2.0%.
CONCLUSION
The results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.
目的
建立一种用高效液相色谱法检测血浆总同型半胱氨酸的方法。
方法
使用Symmetry Shield RP18进行色谱分析。流动相为醋酸钠（0.08 mol/L）和甲醇（1%），我们采用了一种高效液相色谱系统，该系统通过荧光检测与7-氟苯并-2-恶唑-1,3-二氮杂萘-4-磺酸盐（SBD-F）反应衍生化的血浆同型半胱氨酸。
结果
平均回收率为95.8 - 100.8%，相对标准偏差为1.2 - 2.0%。
结论
结果表明该方法是测定血浆中同型半胱氨酸水平的一种快速、准确的方法。
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