Araki A, Sako Y
Department of Geriatric Medicine, Kyoto University School of Medicine, Japan.
J Chromatogr. 1987 Nov 27;422:43-52. doi: 10.1016/0378-4347(87)80438-3.
A simple, sensitive and precise method for the determination of both free and total homocysteine in human plasma is presented. The total homocysteine in plasma consists of free homocysteine (i.e. reduced plus oxidized homocysteine in the non-protein fraction of plasma) and protein-bound homocysteine. The thiol compounds in plasma, which are reduced or liberated from plasma proteins with tri-n-butylphosphine, are derivatized with a thiol-specific fluorogenic reagent, ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate. The derivatives are separated by reversed-phase high-performance liquid chromatography. The concentrations (mean +/- S.D.) of free and total homocysteine in plasma from 35 normal subjects were 1.94 +/- 0.46 and 6.18 +/- 1.19 nmol/ml, respectively.
本文介绍了一种测定人血浆中游离同型半胱氨酸和总同型半胱氨酸的简单、灵敏且精确的方法。血浆中的总同型半胱氨酸由游离同型半胱氨酸（即血浆非蛋白部分中的还原型加氧化型同型半胱氨酸）和与蛋白结合的同型半胱氨酸组成。血浆中的硫醇化合物用三正丁基膦从血浆蛋白中还原或释放出来，然后用硫醇特异性荧光试剂7-氟苯并-2-恶唑-1,3-二氮杂环丁烷-4-磺酸盐进行衍生化。衍生物通过反相高效液相色谱法分离。35名正常受试者血浆中游离同型半胱氨酸和总同型半胱氨酸的浓度（平均值±标准差）分别为1.94±0.46和6.18±1.19 nmol/ml。
