Koga N, Hokama-Kuroki Y, Yoshimura H
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Chem Pharm Bull (Tokyo). 1990 Apr;38(4):1096-7. doi: 10.1248/cpb.38.1096.
Reduction of several nitroso-compounds by purified DT-diaphorase from rat liver cytosol was investigated. Among nitroso-compounds tested, 1-nitroso-2-naphthol and p-nitrosophenol were reduced in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) at rates much faster than that of nitrosobenzene. On the contrary, none of the N-nitroso-compounds tested was reduced by this enzyme. Experiments on the identification of reduction products and on the inhibition with dicoumarol and the antiserum indicated that DT-diaphorase catalyzes 4-electron reduction of C-nitroso-compounds and plays a major role in the reduction of these compounds by rat liver cytosol.
研究了用大鼠肝细胞溶质中纯化的DT-黄递酶对几种亚硝基化合物的还原作用。在所测试的亚硝基化合物中，1-亚硝基-2-萘酚和对亚硝基苯酚在还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在下的还原速度比亚硝基苯快得多。相反，所测试的N-亚硝基化合物均未被该酶还原。对还原产物的鉴定以及用双香豆素和抗血清进行抑制的实验表明，DT-黄递酶催化C-亚硝基化合物的4电子还原，并在大鼠肝细胞溶质对这些化合物的还原中起主要作用。
