Ma Li, Pan Ya-ping, Zhang Jian-quan
Department of Periodontology, China Medical University, Shenyang 110002, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2010 Jun;45(6):337-41.
To detect the putative periodontal pathogens in the subgingival plaque of chronic periodontitis (CP) patients with type 2 diabetes mellitus (DM) and to discuss the role of the periodontal pathogens in the CP and type 2 DM patients.
The study included 154 CP patients with type 2 DM (DM graup), one hundred and twenty subjects with CP (CP group) and 40 healthy subjects. Subgingival plaque samples were obtained with sterile subgingival curettes and pathogens genomic DNA were extracted by phenol and chloroform from plaque. Taking Porphyromonas gingivalis (Pg), Actinobacillus actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), Tannerella forsythia (Tf) and Treponema denticola (Td) as aim pathogens, polymerase chain reaction (PCR) method was used to determine the prevalence of Pg, Aa, Fn, Pi, Tf and Td in the subgingival plaque samples.
Pg, Aa, Fn, Pi, Tf and Td were all found in the subgingival plaque of DM group. In serious periodontitis, the detection rate of Pg, Aa and Tf in DM group [Pg: 78% (72/92), Aa: 27% (25/92), Tf: 67% (62/92)] was significantly higher than in the CP group [Pg: 58% (35/60), Aa: 17% (10/60), Tf: 43% (26/60)], P < 0.05. In slight periodontitis, the detection rate of Pi in DM group was significantly lower than in the CP group. At the same time, the average gradation (AVG) ratio of PCR product of Aa and Tf in DM group was significantly higher than in the CP group and the AVG ratio of Pi in DM group was significantly lower than in the CP group (P < 0.05).
Compared with the pure CP, the quantity of Pg, Aa and Tf in the subgingival plaque of the periodontitis patients with type 2 DM increased, but the Pi quantity reduced.
检测2型糖尿病（DM）合并慢性牙周炎（CP）患者龈下菌斑中的潜在牙周病原体，并探讨牙周病原体在CP及2型DM患者中的作用。
该研究纳入154例2型DM合并CP患者（DM组）、120例CP患者（CP组）和40例健康受试者。用无菌龈下刮治器获取龈下菌斑样本，采用苯酚和氯仿从菌斑中提取病原体基因组DNA。以牙龈卟啉单胞菌（Pg）、伴放线聚集杆菌（Aa）、具核梭杆菌（Fn）、中间普氏菌（Pi）、福赛坦纳菌（Tf）和齿垢密螺旋体（Td）作为目标病原体，采用聚合酶链反应（PCR）法测定龈下菌斑样本中Pg、Aa、Fn、Pi、Tf和Td的检出率。
DM组龈下菌斑中均检测到Pg、Aa、Fn、Pi、Tf和Td。在重度牙周炎中，DM组Pg、Aa和Tf的检出率[Pg：78%（72/92），Aa：27%（25/92），Tf：67%（62/92）]显著高于CP组[Pg：58%（35/60），Aa：17%（10/60），Tf：43%（26/60）]，P<0.05。在轻度牙周炎中，DM组Pi的检出率显著低于CP组。同时，DM组Aa和Tf的PCR产物平均灰度值（AVG）比例显著高于CP组，DM组Pi的AVG比例显著低于CP组（P<０.05）。
与单纯CP相比，2型DM合并牙周炎患者龈下菌斑中Pg、Aa和Tf数量增加，但Pi数量减少。
