Haririan Hady, Andrukhov Oleh, Bertl Kristina, Lettner Stefan, Kierstein Sonja, Moritz Andreas, Rausch-Fan Xiaohui
Division of Conservative Dentistry and Periodontology, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Vienna, Austria.
J Periodontol. 2014 Jun;85(6):819-28. doi: 10.1902/jop.2013.130306. Epub 2013 Oct 21.
The detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. The aim of this cross-sectional study is to investigate whether there is a correlation between the results of different bacterial species in saliva and subgingival plaque samples from individuals with aggressive periodontitis (AgP) and chronic periodontitis (CP).
Whole saliva and subgingival plaque samples from the deepest pocket of each quadrant were collected from 43 patients with CP and 33 patients with AgP. Twenty different bacterial species from both samplings were determined by the 16S ribosomal RNA-based polymerase chain reaction with microarray technique.
All bacterial species were detected in salivary and subgingival plaque samples. For Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, as well as Actinomyces viscosus, Campylobacter rectus/showae, Prevotella intermedia, Parvimonas micra, Eubacterium nodatum, and Campylobacter gracilis, a significant positive correlation between salivary and subgingival plaque samples was detected in patients with both types of periodontitis. There were no significant differences in bacteria in salivary and subgingival plaque samples between AgP and CP.
Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP.
牙周炎患者中特殊细菌种类的检测被认为对临床诊断和治疗有用。龈下菌斑样本的采集是确定牙周病原菌的常用方法。然而，最近，唾液分析作为一种未来牙周炎诊断的优势方法受到了讨论，因为它提供了简单的定量采样以及评估多种细菌的可能性。这项横断面研究的目的是调查侵袭性牙周炎（AgP）和慢性牙周炎（CP）患者唾液和龈下菌斑样本中不同细菌种类的结果之间是否存在相关性。
从43例CP患者和33例AgP患者中采集每个象限最深牙周袋的全唾液和龈下菌斑样本。通过基于16S核糖体RNA的聚合酶链反应与微阵列技术确定两种样本中的20种不同细菌种类。
在唾液和龈下菌斑样本中均检测到所有细菌种类。对于伴放线聚集杆菌、牙龈卟啉单胞菌、具核梭杆菌、福赛坦氏菌，以及黏性放线菌、直肠弯曲菌/昭和弯曲菌、中间普氏菌、微小单胞菌、结节真杆菌和纤细弯曲菌，在两种类型牙周炎患者的唾液和龈下菌斑样本之间检测到显著正相关。AgP和CP患者的唾液和龈下菌斑样本中的细菌没有显著差异。
对于AgP和CP的微生物学分析，唾液分析可能被视为龈下菌斑采样的一种潜在替代方法。
