牙龈卟啉单胞菌不同fimA基因型对口腔上皮细胞黏附与侵袭能力的比较研究
[Comparative study on the ability of adhesion and invasion of different fimA genetypes of Porphyromonas gingivalis to oral epithelial cells].
作者信息
Gao Li, Wu Ya-fei, Miao Di, Zhao Lei, Meng Shu, Wang Jie
机构信息
Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2010 Jun;45(6):342-5.
OBJECTIVE
To compare the ability of adhesion and invasion to epithelial cells by Porphyromonas gingivalis (Pg) strains with different fimA separated from Chinese.
METHODS
Cultured method and antibiotic protection method were used to determine the adhesive and invasive ability of Pg with different fimA genetypes. The adhesion was observed by scanning electron microscope.
RESULTS
All the strains adhered and invaded to KB cells, and the adhesion rate ranged from 0.523% to 37.125% and invasive rate from 0.017% to 3.750%.The adhesive and invasive ability among different fimA genotypes showed no significant difference (P > 0.05).
CONCLUSIONS
There is no significant correlation between fimA genotype and ability in adhesion and invasion to KB cells.
目的
比较从中国人中分离出的不同fimA的牙龈卟啉单胞菌（Pg）菌株对上皮细胞的黏附及侵袭能力。
方法
采用培养法和抗生素保护法测定不同fimA基因型Pg的黏附及侵袭能力。通过扫描电子显微镜观察黏附情况。
结果
所有菌株均能黏附并侵袭KB细胞，黏附率为0.523%至37.125%，侵袭率为0.017%至3.750%。不同fimA基因型之间的黏附及侵袭能力无显著差异（P>0.05）。
结论
fimA基因型与对KB细胞的黏附及侵袭能力之间无显著相关性。
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