Shi Ji, Xue Li-yan, Yin Ning, Lu Jun, Fu Ming, Dong Li-jia, Shao Shu-juan, Tong Tong, Zhan Qi-min
State Key Laboratory of Molecular Oncology, Cancer Hospital and Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
Zhonghua Zhong Liu Za Zhi. 2010 Oct;32(10):748-51.
China has the highest incidence and mortality of esophageal carcinoma in the world, and the esophageal squamous cell carcinoma (ESCC) is the major type. In this study, the authors investigated the expression of Aurora-A in stage T3 esophageal squamous cell carcinomas (ESCC) with positive and negative lymph node metastasis, and analyzed its relationship with prognosis of ESCC patients.
ESCC tissue arrays including 212 specimens had been constructed. The expression of Aurora-A in both ESCC tissues and adjacent normal tissues was determined by immunohistochemical staining. The correlation between Aurora-A protein levels and lymph node status in ESCC and survival rate were statistically analyzed.
The positive expression of Aurora-A was 74.07% (140/189) in tumor tissues and 18.52% (35/189) in adjacent normal tissues, showing a significant difference between them (χ(2) = 105.162, P < 0.05). In tumors with positive lymph nodes, strong positive expression of Aurora-A was found in 42.99% (46/107), while only 7.37% (7/95) in tumors with negative lymph nodes, with a statistically significant difference (χ(2) = 36.132, P < 0.05). The cumulative survival rate of the patients with strong Aurora-A-positive tumors was significantly lower than that in patients with Aurora-A-negative tumors (P = 0.0042, P < 0.05).
The positive expression of Aurora-A in ESCC tissues is much higher than that in adjacent normal tissues. The expression of Aurora-A is higher in lymph node-positive tumors than in the lymph node-negative ones. There is a significantly longer cumulative survival rate in patients with negative Aurora-A expression than that in patients with strong positive Aurora-A expression.
中国是世界上食管癌发病率和死亡率最高的国家，食管鳞状细胞癌（ESCC）是主要类型。在本研究中，作者调查了Aurora-A在伴有阳性和阴性淋巴结转移的T3期食管鳞状细胞癌（ESCC）中的表达，并分析其与ESCC患者预后的关系。
构建了包含212个标本的ESCC组织芯片。通过免疫组织化学染色确定ESCC组织和相邻正常组织中Aurora-A的表达。对ESCC中Aurora-A蛋白水平与淋巴结状态及生存率之间的相关性进行统计学分析。
Aurora-A在肿瘤组织中的阳性表达率为74.07%（140/189），在相邻正常组织中的阳性表达率为18.52%（35/189），两者之间存在显著差异（χ(2)=105.162，P<0.05）。在淋巴结阳性的肿瘤中，42.99%（46/107）的肿瘤Aurora-A呈强阳性表达，而在淋巴结阴性的肿瘤中，只有7.37%（7/95）呈强阳性表达，差异有统计学意义（χ(2)=36.132，P<0.05）。Aurora-A强阳性肿瘤患者的累积生存率显著低于Aurora-A阴性肿瘤患者（P=0.0042，P<0.05）。
ESCC组织中Aurora-A的阳性表达远高于相邻正常组织。Aurora-A在淋巴结阳性肿瘤中的表达高于淋巴结阴性肿瘤。Aurora-A表达阴性的患者累积生存率明显长于Aurora-A强阳性表达的患者。
