Sun Xiao-hong, Zhang Xiao-long, Liu Yu-fu, Wang Jing, Wei Lian, Gao Xu, Zhao Wen, Zhang Xun, Yang Xue-bing, Zhang Le
Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jun;31(6):685-7.
To isolate and identify Banna virus (BAV) from mosquitoes collected in Mengla county of Yunnan province.
Mosquito samples were collected in houses and stock yards in Mengla county, 2008. Mosquitoes were homogenized and incubated onto both C6/36 and BHK21 cells. The new isolate was identified by using ELISA and RT-PCR. The sequences of segment 5, 8 and 11 of BAV were amplified by RT-PCR and determined. Phylogenetic analysis on the new BAV were performed using MEGA4 program.
1731 mosquitoes representing 7 species were collected with one strain of BAV isolated and identified. Phylogenetic analysis based on sequences of segment 8 showed the new isolate was closed to BAV strain isolated in Yunnan, but segment 11 sequence was closed to Vietnam strain.
Results of phylogenetic analysis implied that the BAV re-assortment might have been occurred both in Chinese and Vietnam strains.
从云南省勐腊县采集的蚊虫中分离并鉴定版纳病毒（BAV）。
2008年在勐腊县的房屋和牲畜圈舍采集蚊虫样本。将蚊虫匀浆后接种于C6/36和BHK21细胞。采用酶联免疫吸附测定法（ELISA）和逆转录-聚合酶链反应（RT-PCR）对新分离株进行鉴定。通过RT-PCR扩增并测定BAV第5、8和11节段的序列。使用MEGA4软件对新的BAV进行系统发育分析。
共采集了代表7个蚊种的1731只蚊虫，分离并鉴定出1株BAV。基于第8节段序列的系统发育分析表明，新分离株与云南分离的BAV株相近，但第11节段序列与越南株相近。
系统发育分析结果提示，中国株和越南株的BAV可能均发生了重配。
