Tao S, Cai Z, Yang D
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1999 Sep 30;13(3):228-30.
The purpose of this study was to isolate new arbovirus from mosquitoes collected from the northeast part of China during the summer of 1996.
The cell used for isolation were C6/36 and BHK-21 cells.
Two strains (NE97-12 and NE97-31) of coltivirus were isolated among 68 mosquito specimens. The viruses caused cytopathogenic effects on C6/36 cell, but not on BHK-21 cells. They were resistant to both 5-IDU and ether, but sensitive to pH3.0 and temperature at 56 degrees C for 30 minutes. IFA of new isolates with mouse ascitic fluid anti-coltivirus TRT2 strain showed positive reaction. Polyacrylamide gel electrophoresis (PAGE) of two isolates showed 12 segments of double-stranded(ds) RNA and PAGE profiles were 6-5-1, which were different from that of coltivirus TRT2 strain(6-6). There was no cross neutralization between new isolates and TRT2 strain by tissue culture cross-neutralization test.
The results indicated while new isolates were serologically related to TRT2 strain of coltivirus by IFA, the neutralization test suggests that it represents a distinct serotype. These new isolates isolated firstly from the northeast part of China belong to a new subtype of coltivirus.
本研究旨在从1996年夏季在中国东北地区采集的蚊子中分离新的虫媒病毒。
用于分离的细胞是C6/36和BHK - 21细胞。
在68个蚊子标本中分离出两株科蒂病毒（NE97 - 12和NE97 - 31）。这些病毒对C6/36细胞产生细胞病变效应，但对BHK - 21细胞无此效应。它们对5 - IDU和乙醚均有抗性，但对pH3.0和56℃温度30分钟敏感。用小鼠腹水抗科蒂病毒TRT2株对新分离株进行间接免疫荧光法（IFA）检测显示阳性反应。两株分离株的聚丙烯酰胺凝胶电泳（PAGE）显示有12条双链（ds）RNA片段，PAGE图谱为6 - 5 - 1，与科蒂病毒TRT2株（6 - 6）不同。通过组织培养交叉中和试验，新分离株与TRT2株之间无交叉中和作用。
结果表明，虽然通过IFA新分离株在血清学上与科蒂病毒TRT2株相关，但中和试验表明它代表一个独特的血清型。这些首次从中国东北地区分离的新分离株属于科蒂病毒的一个新亚型。
