Beijing Tropical Disease Institute, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Chin Med J (Engl). 2010 Nov;123(22):3247-51.
In our previous studies, we found the expression of 14-kD phosphohistidine phosphatase (PHPT1) was associated with lung cancer cells migration and invasion, and PHPT1 mRNA expression level in lung cancer tissues clinically correlated with lymph node metastasis. In the present study, we aimed to further investigate the expression of PHPT1 protein in lung cancer.
Expression of PHPT1 protein in tissue samples from 146 lung cancers and 30 normal tissues adjacent to lung cancers was assessed using immunohistochemical method. Fisher's exact test was used to analyze expression patterns of PHPT1 protein in these tissue types. Meanwhile, we studied the correlation between expression of PHPT1 protein and clinicopathological features in lung cancer.
Significantly higher expression levels of PHPT1 protein were found in lung cancer samples (53.42%) than in normal tissues adjacent to lung cancer (23.33%) (P = 0.003). Fisher's exact test showed that lung cancer stage positively correlated with expression of PHPT1 protein (P = 0.02), and lung cancer samples with lymph node metastasis showed higher PHPT1 protein expression (P = 0.016) than the samples without lymph node metastasis.
The results of this study agree with findings from our previous study of PHPT1 mRNA expression in lung cancer tissues, and strongly suggest that PHPT1 protein is closely associated with the carcinogenesis and metastasis of lung cancer. Thus, therapy targeting PHPT1 (inhibition or silencing) could be potentially benefited for lung cancer patients.
在我们之前的研究中，我们发现 14-kD 磷酸组氨酸磷酸酶（PHPT1）的表达与肺癌细胞的迁移和侵袭有关，并且肺癌组织中 PHPT1 mRNA 的表达水平与淋巴结转移临床相关。在本研究中，我们旨在进一步研究 PHPT1 蛋白在肺癌中的表达。
采用免疫组织化学方法检测 146 例肺癌组织和 30 例肺癌旁正常组织中 PHPT1 蛋白的表达。采用 Fisher 确切检验分析这些组织类型中 PHPT1 蛋白的表达模式。同时，我们研究了 PHPT1 蛋白表达与肺癌临床病理特征的相关性。
肺癌组织中 PHPT1 蛋白的表达水平明显高于肺癌旁正常组织（53.42%比 23.33%）（P = 0.003）。Fisher 确切检验显示，肺癌分期与 PHPT1 蛋白的表达呈正相关（P = 0.02），并且有淋巴结转移的肺癌样本中 PHPT1 蛋白的表达高于无淋巴结转移的样本（P = 0.016）。
本研究结果与我们之前对肺癌组织中 PHPT1 mRNA 表达的研究结果一致，强烈提示 PHPT1 蛋白与肺癌的发生和转移密切相关。因此，针对 PHPT1 的治疗（抑制或沉默）可能对肺癌患者有益。
