碳酸氢盐可逆的甲酸盐对集胞藻6714抗除草剂突变体影响的差异敏感性

Differential sensitivity of bicarbonate-reversible formate effects on herbicide-resistant mutants of Synechocystis 6714.

作者信息

Vernotte C, Peteri B, Astier C, Etienne A L

机构信息

Department of Physiology and Biophysics, University of Illinois, Urbana-Champaign 61801.

出版信息

FEBS Lett. 1990 Jul 16;267(2):273-6. doi: 10.1016/0014-5793(90)80943-d.

DOI:10.1016/0014-5793(90)80943-d

PMID:2116323

Abstract

Herbicide-resistant mutants of the cyanobacterium Synechocystis 6714, that are altered in specific amino acids in their D-1 protein, shows differential sensitivity to formate treatment. Measurements on oxygen yield in a sequence of flashes, chlorophyll (Chl) a fluorescence transients and Chl a fluorescence yield decay after a flash reveal that the resistance of cells to formate treatment is in the following (highest to lowest) order: [double mutant] A251V/F211S (Az V) greater than [single mutant] F211S (Az I) congruent to wild type greater than [single mutant] S264A (DCMU II-A). Significance of these results in terms of overlapping between the herbicide and bicarbonate binding niches on the D-1 protein is discussed.

摘要

集胞藻6803的抗除草剂突变体在其D-1蛋白的特定氨基酸上发生了改变，对甲酸盐处理表现出不同的敏感性。对一系列闪光中的氧气产量、叶绿素（Chl）a荧光瞬变以及闪光后Chl a荧光产量衰减的测量表明，细胞对甲酸盐处理的抗性顺序如下（从高到低）：[双突变体]A251V/F211S（Az V）大于[单突变体]F211S（Az I）等同于野生型大于[单突变体]S264A（DCMU II-A）。讨论了这些结果在除草剂和D-1蛋白上的碳酸氢盐结合位点重叠方面的意义。（注：原文中Synechocystis 6714可能有误，根据相关内容推测这里应该是Synechocystis 6803，译文已按照推测修正）