Creuzet S, Ajlani G, Vernotte C, Astier C
Dept. de Biologie, CEA/INRA Saclay, Gif sur Yvette, France.
Z Naturforsch C J Biosci. 1990 May;45(5):436-40. doi: 10.1515/znc-1990-0521.
A new Synechocystis 6714 mutant, IoxIIA, resistant to the phenol-type herbicide ioxynil was isolated and characterized. The mutation found in the psbA gene (encoding the D 1 photosystem II protein) is at the same codon 266 as for the first ioxynil-resistant mutant IoxIA previously selected [G. Ajlani, I. Meyer, C. Vernotte, and C. Astier, FEBS Lett. 246, 207-210 (1989)]. In IoxIIA, the change of Asn 266 to Asp gives a 3 x resistance, whereas in IoxIA, the change of the same amino acid to Thr gives a 10 x resistance. The effect of these different amino acid substitutions on the ioxynil resistance phenotype has allowed us to construct molecular models and calculate the hydrogen-bonding energies between the hydroxyl group of ioxynil and the respective amino acids at position 266.
分离并鉴定了一种新的集胞藻6714突变体IoxIIA，它对酚类除草剂碘苯腈具有抗性。在psbA基因（编码光系统II的D1蛋白）中发现的突变与先前筛选出的第一个抗碘苯腈突变体IoxIA位于相同的密码子266处[G. Ajlani, I. Meyer, C. Vernotte, and C. Astier, FEBS Lett. 246, 207 - 210 (1989)]。在IoxIIA中，第266位的天冬酰胺变为天冬氨酸产生了3倍抗性，而在IoxIA中，相同氨基酸变为苏氨酸产生了10倍抗性。这些不同的氨基酸取代对碘苯腈抗性表型的影响使我们能够构建分子模型，并计算碘苯腈羟基与第266位相应氨基酸之间的氢键能。
