Enteric Viruses Department, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune - 411 001, India.
Microbes Infect. 2011 Apr;13(4):331-8. doi: 10.1016/j.micinf.2010.12.001. Epub 2010 Dec 14.
Experimental studies of human rotavirus infections in mice are limited and there is lack of information on the quantitative assessment of rotaviral replication and its relationship with histological changes. In the present study, consequences of human rotavirus strain, YO induced gastroenteritis in infant BALB/c mice were analyzed for the occurrence of clinical symptoms, histopathology and virological events. The infected animals developed diarrhea and dehydration and showed accumulation of vacuolated enterocytes with lodging of the rotavirus antigens and shortening of villi in the intestine over a period of 5 days. The ileum was identified as the most susceptible and supportive part of small intestine for perpetuation of rotavirus infection in mice. Rotaviral antigen/RNA in stool and RNA in intestine were detected throughout the clinical disease period. At 48-72 h post inoculation, diarrhea was at the peak (90-95%) in the infected animals with increased load of viral RNA and intense pathological lesions suggesting it as the critical time point in the course of infection. The rising titers of antirotavirus neutralizing antibodies ascertained the replication of human rotavirus strain, YO in mice. These data may contribute to the understanding of pathophysiological, immunological and virological characteristics of rotavirus infections in mice.
在小鼠中进行人类轮状病毒感染的实验研究受到限制，缺乏关于轮状病毒复制的定量评估及其与组织学变化关系的信息。在本研究中，分析了人轮状病毒株 YO 诱导的婴儿 BALB/c 小鼠胃肠炎的临床症状、组织病理学和病毒学事件的后果。受感染的动物在 5 天的时间内出现腹泻和脱水，并在肠道中出现空泡化肠细胞堆积，轮状病毒抗原和绒毛缩短。回肠被确定为小鼠轮状病毒感染持续存在的最易感和支持小肠部分。轮状病毒抗原/粪便中的 RNA 和肠道中的 RNA 在整个临床疾病期间均被检测到。接种后 48-72 小时，感染动物的腹泻达到高峰（90-95%），病毒 RNA 载量增加，病理损伤强烈，表明这是感染过程中的关键时间点。抗轮状病毒中和抗体滴度的升高证实了人轮状病毒株 YO 在小鼠中的复制。这些数据可能有助于理解轮状病毒感染在小鼠中的生理病理、免疫和病毒学特征。
