Kikkoman USA R&D Laboratory Inc., 505 South Rosa Road, Suite 107, Madison, WI 53719, USA.
Appl Physiol Nutr Metab. 2010 Dec;35(6):749-54. doi: 10.1139/H10-069.
Wheel running by rodents is thought to reflect voluntary exercise in humans. The present study examined the effect of fermented soymilk (FSM) on voluntary wheel running in rats. FSM was prepared from soymilk (SM) using the bacteria Leuconostoc pseudomesenteroides. The rats were fed a normal diet for 3 weeks followed by a 3-week administration of diet containing FSM or SM (5% w/w), and then the diets were switched back to a normal diet for 3 weeks. The voluntary wheel running activity was increased by FSM administration, although no changes were observed by SM administration. This effect was observed 2 weeks after FSM administration and lasted 1 week after deprivation of FSM. Then we evaluated the effect of FSM on sexual behavior in male rats. FSM administration for 10 days significantly increased the number of mounts. The protein expression of tyrosine hydroxylase (TH) increased in the hippocampus by FSM administration and it is suggested that FSM may change norepinephrine or dopamine signaling in the brain. Our study provides the first evidence that FSM increases voluntary wheel running activity and sexual behavior and suggests that TH may be involved in these effects.
啮齿动物的转轮行为被认为反映了人类的自愿运动。本研究探讨了发酵豆浆（FSM）对大鼠自愿转轮行为的影响。FSM 是通过使用 Leuconostoc pseudomesenteroides 细菌从豆浆（SM）中制备的。大鼠在正常饮食下喂养 3 周，然后用含有 FSM 或 SM（5%w/w）的饮食进行 3 周的管理，然后再切换回正常饮食 3 周。FSM 的给予增加了大鼠的自愿转轮活动，而 SM 的给予则没有观察到变化。这种作用在 FSM 给予后 2 周观察到，并在剥夺 FSM 后持续 1 周。然后，我们评估了 FSM 对雄性大鼠性行为的影响。FSM 给药 10 天可显著增加交配次数。FSM 给药增加了海马体中酪氨酸羟化酶（TH）的蛋白表达，表明 FSM 可能改变大脑中的去甲肾上腺素或多巴胺信号。我们的研究首次提供了 FSM 增加自愿转轮活动和性行为的证据，并表明 TH 可能参与这些作用。
