Galli J, Lendahl U, Paulsson G, Ericsson C, Bergman T, Carlquist M, Wieslander L
Department of Molecular Genetics, Karolinska Institutet, Stockholm, Sweden.
J Mol Evol. 1990 Jul;31(1):40-50. doi: 10.1007/BF02101791.
We describe the structure of a gene expressed in the salivary gland cells of the dipteran Chironomus tentans and show that it encodes 1 of the approximately 15 secretory proteins exported by the gland cells. This sp115,140 gene consists of approximately 65 copies of a 42-bp sequence in a central uninterrupted core block, surrounded by short nonrepetitive regions. The repeats within the gene are highly similar to each other, but divergent repeats are present in a pattern which suggests that the repeat structure has been remodeled during evolution. The 42-bp repeat in the gene is a simple variant of the more complex repeat unit present in the Balbiani ring genes, encoding four of the other secretory proteins. The structure of the sp115,140 gene suggests that related repeat structures have evolved from a common origin and resulted in the set of genes whose secretory proteins interact in the assembly of the secreted protein fibers.
我们描述了在双翅目昆虫摇蚊唾腺细胞中表达的一个基因的结构，并表明它编码由腺细胞输出的大约15种分泌蛋白中的一种。这个sp115,140基因在一个中央不间断的核心区域由大约65个42碱基对的序列组成，周围是短的非重复区域。基因内的重复序列彼此高度相似，但存在分歧的重复序列，其模式表明重复结构在进化过程中已经重塑。基因中的42碱基对重复序列是巴尔比亚尼环基因中存在的更复杂重复单元的一个简单变体，后者编码其他四种分泌蛋白。sp115,140基因的结构表明相关的重复结构从一个共同的起源进化而来，并产生了一组基因，其分泌蛋白在分泌蛋白纤维的组装中相互作用。
