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摇蚊唾液腺最小分泌蛋白基因的结构

Structure of the smallest salivary-gland secretory protein gene in Chironomus tentans.

作者信息

Galli J, Wieslander L

机构信息

Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.

出版信息

J Mol Evol. 1994 May;38(5):482-8. doi: 10.1007/BF00178848.

DOI:10.1007/BF00178848
PMID:8028027
Abstract

The salivary gland secretion in the dipteran Chironomus tentans is composed of approximately 15 different secretory proteins. The most well known of the corresponding genes are the four closely related Balbiani ring (BR) genes, in which the main part of each approximately 40-kb gene is composed of tandemly arranged repetitive units. Six of the seven additional secretory protein genes described share structural similarities with the BR genes and are members of the same BR multigene family. Here we report the identification of a new secretory protein gene, the sp12 gene, encoding the smallest component of the C. tentans salivary gland secretion. The gene has a corresponding mRNA length of approximately 0.7 kb and codes for a protein with a calculated molecular weight of 7,619 Da. The sp12 gene was characterized in seven Chironomus species. Based on a comparison of the orthologous gene sequences, we conclude that the sp12 gene has a repetitive structure consisting of diverged 21-bp-long repeats. The repeat structure and the codon composition are similar to the so-called SR regions of the BR genes and the sp12 gene may represent a diverged member of the BR multigene family.

摘要

双翅目昆虫摇蚊的唾液腺分泌物由大约15种不同的分泌蛋白组成。相应基因中最著名的是四个紧密相关的巴尔比亚尼环（BR）基因，每个大约40 kb的基因的主要部分由串联排列的重复单元组成。所描述的另外七个分泌蛋白基因中有六个与BR基因具有结构相似性，并且是同一BR多基因家族的成员。在此，我们报告了一个新的分泌蛋白基因sp12的鉴定，该基因编码摇蚊唾液腺分泌物中最小的成分。该基因的相应mRNA长度约为0.7 kb，编码一种计算分子量为7619 Da的蛋白质。sp12基因在七种摇蚊物种中得到了表征。基于直系同源基因序列的比较，我们得出结论，sp12基因具有由21 bp长的分歧重复序列组成的重复结构。该重复结构和密码子组成与BR基因的所谓SR区域相似，并且sp12基因可能代表BR多基因家族的一个分歧成员。

相似文献

1
Structure of the smallest salivary-gland secretory protein gene in Chironomus tentans.摇蚊唾液腺最小分泌蛋白基因的结构
J Mol Evol. 1994 May;38(5):482-8. doi: 10.1007/BF00178848.
2
A new member of the balbiani ring multigene family in the dipteran Chironomus tentans consists of a single-copy version of a unit repeated in other gene family members.双翅目昆虫摇蚊（Chironomus tentans）中巴尔比亚尼环多基因家族的一个新成员，由在其他基因家族成员中重复的一个单元的单拷贝版本组成。
J Mol Evol. 1993 Nov;37(5):457-63. doi: 10.1007/BF00160426.
3
The Balbiani ring 3 gene in Chironomus tentans has a diverged repetitive structure split by many introns.摇蚊的巴尔比亚尼环3基因具有由许多内含子分割的分化重复结构。
J Mol Biol. 1990 Jan 20;211(2):331-49. doi: 10.1016/0022-2836(90)90355-P.
4
A repetitive secretory protein gene of a novel type in Chironomus tentans is specifically expressed in the salivary glands and exhibits extensive length polymorphism.摇蚊（Chironomus tentans）中一种新型的重复分泌蛋白基因在唾液腺中特异性表达，并表现出广泛的长度多态性。
J Biol Chem. 1993 Jun 5;268(16):11888-93.
5
A new member of a secretory protein gene family in the dipteran Chironomus tentans has a variant repeat structure.双翅目昆虫摇蚊的一个分泌蛋白基因家族的新成员具有变异的重复结构。
J Mol Evol. 1990 Jul;31(1):40-50. doi: 10.1007/BF02101791.
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Two secretory protein genes in Chironomus tentans have arisen by gene duplication and exhibit different developmental expression patterns.摇蚊的两个分泌蛋白基因通过基因复制产生，并表现出不同的发育表达模式。
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Balbiani ring 1 gene in Chironomus tentans. Sequence organization and dynamics of a coding minisatellite.摇蚊Balbiani环1基因。编码小卫星的序列组织与动态变化
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Correlated changes in steady-state levels of Balbiani ring mRNAs and secretory polypeptides in salivary glands of Chironomus tentans.摇蚊唾腺中巴尔比亚尼环信使核糖核酸和分泌性多肽稳态水平的相关变化。
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Identification of a developmentally regulated gene for a 140-kDa secretory protein in salivary glands of Chironomus tentans larvae.摇蚊幼虫唾液腺中一种140 kDa分泌蛋白的发育调控基因的鉴定。
J Biol Chem. 1989 Jun 5;264(16):9444-52.

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本文引用的文献

1
[Chromomore constancy and specific modifications of the chromosome structure in development and organ differentiation of Chironomus tentans].[摇蚊发育和器官分化过程中染色体组的稳定性及染色体结构的特异性修饰]
Chromosoma. 1952;5(2).
2
Two secretory protein genes in Chironomus tentans have arisen by gene duplication and exhibit different developmental expression patterns.摇蚊的两个分泌蛋白基因通过基因复制产生，并表现出不同的发育表达模式。
J Mol Biol. 1993 May 20;231(2):324-34. doi: 10.1006/jmbi.1993.1285.
3
A repetitive secretory protein gene of a novel type in Chironomus tentans is specifically expressed in the salivary glands and exhibits extensive length polymorphism.
摇蚊（Chironomus tentans）中一种新型的重复分泌蛋白基因在唾液腺中特异性表达，并表现出广泛的长度多态性。
J Biol Chem. 1993 Jun 5;268(16):11888-93.
4
Chironomus tentans epithelial cell lines sensitive to ecdysteroids, juvenile hormone, insulin and heat shock.摇蚊上皮细胞系对蜕皮激素、保幼激素、胰岛素和热休克敏感。
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Determination of the developmental stage of living fourth instar larvae of Chironomus tentans.摇蚊四龄幼虫发育阶段的确定。
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
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Balbiani ring DNA: sequence comparisons and evolutionary history of a family of hierarchically repetitive protein-coding genes.巴尔比亚尼环DNA：一个分层重复蛋白质编码基因家族的序列比较与进化史
J Mol Evol. 1984;20(3-4):281-95. doi: 10.1007/BF02104734.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
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9
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”：蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上，并用抗体和放射性碘化蛋白A进行放射自显影检测。
Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
10
Dependence of Balbiani ring induction in Chironomus salivary glands on inorganic phosphate.
Dev Biol. 1982 May;91(1):131-7. doi: 10.1016/0012-1606(82)90016-1.