淋病奈瑟菌和其他奈瑟菌属菌种菌株中淋球菌脂多糖生物合成基因的分布。

Distribution of gonococcal lipopolysaccharide biosynthesis genes among strains of Neisseria gonorrhoeae and other neisserial species.

作者信息

Palermo-Dilts D A, Silver L E, Clark V L

机构信息

Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, NY 14642.

出版信息

Microb Pathog. 1990 Mar;8(3):227-33. doi: 10.1016/0882-4010(90)90049-v.

DOI:10.1016/0882-4010(90)90049-v

PMID:2116567

Abstract

A plasmid, pTME6, containing Neisseria gonorrhoeae lipopolysaccharide biosynthesis genes was used as a probe to analyze DNA from strains of N. gonorrhoeae, N. meningitidis and various commensal Neisseria by Southern blotting. Chromosomal DNA from 26 gonococcal strains probed with 32P-labeled pTME6 produced five different hybridization patterns. No correlation between hybridization pattern and auxotype, serotype, serum sensitivity or SDS-urea-PAGE migration of LPS was observed. DNA from strains of N. meningitidis, N. lactamica and N. cinerea, but not other commensal Neisseria species, hybridized strongly to pTME6.

摘要

一个含有淋病奈瑟菌脂多糖生物合成基因的质粒pTME6被用作探针，通过Southern印迹法分析淋病奈瑟菌、脑膜炎奈瑟菌菌株以及各种共生奈瑟菌的DNA。用32P标记的pTME6探测26株淋球菌的染色体DNA，产生了五种不同的杂交模式。未观察到杂交模式与营养缺陷型、血清型、血清敏感性或脂多糖的SDS-尿素-聚丙烯酰胺凝胶电泳迁移率之间存在相关性。脑膜炎奈瑟菌、乳酸奈瑟菌和灰色奈瑟菌菌株的DNA能与pTME6强烈杂交，但其他共生奈瑟菌物种的DNA则不能。