Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Indian J Pediatr. 2011 Apr;78(4):478-81. doi: 10.1007/s12098-010-0309-5. Epub 2010 Dec 17.
Viral infections may have an important role in the pathogenesis of biliary atresia, and related clinical outcomes. In this research for determination of the possible role of HBV, HCV, HCMV, adenovirus, and BK virus infections in biliary atresia related clinical complications, the molecular and antigenic prevalence of these viral agents were studied. In this retrospective study, 34 formalin fixed paraffin embedded (FFPE) biopsy and autopsy liver tissue samples of neonates with biliary atresia were evaluated. The molecular prevalence of these viral infections was assayed by different PCR and RT-PCR methods. The antigenic prevalence of HBV, HCV, and HCMV infections was also studied in these liver tissue samples by immunohistochemistry (IHC) method. HBV, HCV, and adenovirus genomes were detected in 9%, 6%, and 6% of liver autopsy and biopsy tissues of infants with biliary atresia, respectively. HBV and HCV co-infection was confirmed in 6% of FFPE samples. The genome of other investigated viruses was not detected in FFPE liver tissues. Detection of viral infection in FFPE liver tissue samples of newborns with biliary atresia, suggests the need for complete studies for the determination of accurate role of these viral infections in pathogenesis of biliary atresia.
病毒感染可能在胆道闭锁的发病机制和相关临床结局中起重要作用。在这项研究中，我们旨在确定乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）、巨细胞病毒（HCMV）、腺病毒和 BK 病毒感染在胆道闭锁相关临床并发症中的可能作用，研究了这些病毒因子的分子和抗原流行情况。在这项回顾性研究中，我们评估了 34 例福尔马林固定石蜡包埋（FFPE）的胆道闭锁新生儿活检和尸检肝组织样本。通过不同的 PCR 和 RT-PCR 方法检测这些病毒感染的分子流行情况。通过免疫组织化学（IHC）方法研究了这些肝组织样本中 HBV、HCV 和 HCMV 感染的抗原流行情况。HBV、HCV 和腺病毒基因组分别在胆道闭锁婴儿的肝尸检和活检组织中检测到 9%、6%和 6%。在 6%的 FFPE 样本中证实了 HBV 和 HCV 合并感染。其他研究病毒的基因组未在 FFPE 肝组织中检测到。在胆道闭锁新生儿的 FFPE 肝组织样本中检测到病毒感染，表明需要进行完整的研究，以确定这些病毒感染在胆道闭锁发病机制中的准确作用。
