Minimally Invasive Surgery Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Arch Gynecol Obstet. 2011 Nov;284(5):1157-62. doi: 10.1007/s00404-010-1806-5. Epub 2010 Dec 17.
Recent studies indicated that there is a high density of small nerve fibers in the functional layer of the endometrium in women with endometriosis and that it can be used as a marker to detect endometriosis. In this study, the efficacy assessment of small nerve fibers' density as a diagnostic marker was compared in patients with and without endometriosis.
In this study, women with history of pelvic pain and/or infertility who were candidates for laparoscopy or laparotomy in Rassoul hospital (2007-2009) were enrolled. Histological sections of endometrial tissue were prepared from endometrial biopsy from women with endometriosis (n = 12) (1) and without endometriosis (n = 15) (2). Protein gene product 9.5 and neurofilament were evaluated as marker from endometrial biopsies by immunohistochemical methods.
There was no statistically significant difference between two groups according to age, body mass index. Nerve fibers were detected in all endometrial biopsies from all women with endometriosis but detected only in three women without endometriosis. The mean density of nerve fibers was 2.2 ± 4.7 mm(-2) in group without endometriosis and) 13.1 ± 3.3 (in group with endometriosis (p < 0.001). Women with endometriosis had significantly higher nerve fiber density in comparison with women without endometriosis.
Our findings indicated that endometrial biopsy for detecting density of nerve fibers by usage of protein gene product 9.5, provided a reliable marker for diagnosis of endometriosis.
最近的研究表明，子宫内膜功能层中存在高密度的小神经纤维，可作为诊断子宫内膜异位症的标志物。本研究旨在比较小神经纤维密度作为诊断标志物在子宫内膜异位症患者和非子宫内膜异位症患者中的诊断效能。
本研究纳入了 2007 年至 2009 年在拉苏尔医院因盆腔疼痛和/或不孕而接受腹腔镜或剖腹手术的患者。从子宫内膜异位症患者（n=12，组 1）和非子宫内膜异位症患者（n=15，组 2）的子宫内膜活检中制备子宫内膜组织的组织学切片。通过免疫组织化学方法评估蛋白基因产物 9.5 和神经丝作为子宫内膜活检的标志物。
两组患者的年龄、体重指数无统计学差异。所有子宫内膜异位症患者的子宫内膜活检中均检测到神经纤维，但在非子宫内膜异位症患者中仅检测到 3 例。无子宫内膜异位症患者的神经纤维密度平均值为 2.2±4.7mm-2，而子宫内膜异位症患者为 13.1±3.3（p<0.001）。与无子宫内膜异位症患者相比，子宫内膜异位症患者的神经纤维密度明显更高。
本研究结果表明，通过使用蛋白基因产物 9.5 检测神经纤维密度的子宫内膜活检为子宫内膜异位症的诊断提供了可靠的标志物。
