Cetin Cihan, Serdaroglu Hasan, Tuzlali Sitki
Department of Obstetrics and Gynecology, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey.
Department of Obstetrics and Gynecology, Reproductive Endocrinology and Infertility Unit, Istanbul School of Medicine, Istanbul University, Istanbul, Turkey.
Iran J Reprod Med. 2013 May;11(5):405-14.
Endometriosis is a disease that is hard to diagnose without the gold standard method, laparoscopy. An easier diagnostic method is needed.
The aim of the study is to determine whether the number of macrophage cells in the endometrium and/or the detection of nerve fibers can be used in the diagnosis of endometriosis.
Endometrial sampling was done to 31 patients prior to laparoscopy (L/S) or laparotomy (L/T) at Istanbul University Istanbul School of Medicine Hospital between January 2010 February 2011. Also 34 patients who were retrospectively chosen from their files were added to the study. 5 patients were excluded from the study. Totally, 31 patients were placed in the endometriosis and 29 patients in the control group. Endometrial samples were evaluated immunohistochemically with the markers protein gene product 9.5 (PGP 9.5) and neurofilament (NF) for nerve fibers and CD68 for macrophages.
None of the samples were stained with PGP 9.5 and NF. As for CD68+cells, no statistically significant difference was observed between groups (endometriosis: 216.10±104.41; control: 175.93±43.05, p=0.06). RESULTS were also evaluated in the subgroups of menstruel phases and disease stages. Only in the proliferative phase there was a significant increase in the endometriosis group (p=0.03). No significant difference was observed between the stages.
The detection of nerve fibers in the eutopic endometrium with the markers of PGP 9.5 and NF is not found to be helpful in the diagnosis of endometriosis. Macrophage cells may be helpful in the diagnosis only in the proliferative phase.
子宫内膜异位症是一种没有金标准方法（腹腔镜检查）就难以诊断的疾病。需要一种更简便的诊断方法。
本研究的目的是确定子宫内膜中巨噬细胞的数量和/或神经纤维的检测是否可用于子宫内膜异位症的诊断。
2010年1月至2011年2月期间，在伊斯坦布尔大学伊斯坦布尔医学院医院对31例患者在腹腔镜检查（L/S）或剖腹手术（L/T）前进行了子宫内膜采样。另外，从病历中回顾性选取的34例患者也加入了研究。5例患者被排除在研究之外。总共，31例患者被纳入子宫内膜异位症组，29例患者被纳入对照组。用蛋白基因产物9.5（PGP 9.5）和神经丝（NF）标记神经纤维，用CD68标记巨噬细胞，对子宫内膜样本进行免疫组织化学评估。
所有样本均未被PGP 9.5和NF染色。至于CD68+细胞，两组之间未观察到统计学上的显著差异（子宫内膜异位症组：216.10±104.41；对照组：175.93±43.05，p = 0.06）。结果还在月经周期和疾病分期的亚组中进行了评估。仅在增殖期，子宫内膜异位症组有显著增加（p = 0.03）。各期之间未观察到显著差异。
未发现用PGP 9.5和NF标记检测在位子宫内膜中的神经纤维对子宫内膜异位症的诊断有帮助。巨噬细胞可能仅在增殖期对诊断有帮助。
