Department of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Histopathology. 2010 Dec;57(6):825-35. doi: 10.1111/j.1365-2559.2010.03717.x.
Autoimmune pancreatitis (AIP) is a type of pancreatitis whose immunopathogenesis is still unknown. It has been reported that renal biopsy specimens from patients diagnosed with both AIP and tubulointerstitial nephritis reveal deposits containing complement C3, immunoglobulin (Ig)G and IgG4 at the tubular basement membranes (BMs). The aim was to investigate the deposition of complement and immunoglobulins in pancreatic tissue from AIP patients compared to non-AIP patients.
Double immunofluorescence microscopy for C3c, IgG4 and IgG together with CK7, trypsin, collagen IV, CD31 and CD79a, as well as immunofluorescence microscopy for C1q, IgA and IgM, were performed on frozen pancreatic tissue from AIP and alcoholic chronic pancreatitis (ACP) patients.
In AIP patients, complement C3c, IgG4 and IgG were deposited at the collagen IV-positive BMs of pancreatic and bile ducts and of acini. In a minority of the ACP patients, weak C3c-positive BM deposits were detected, but no IgG4- or IgG-positive BM deposits were present.
The deposition of C3c, IgG4 and IgG at the BM of small- and medium-sized ducts and acini of the pancreas is characteristic of AIP. This suggests that immune complex-mediated destruction of ducts and acini play a role in the pathogenesis of AIP.
自身免疫性胰腺炎（AIP）是一种胰腺炎，其免疫发病机制尚不清楚。据报道，同时诊断为 AIP 和肾小管间质性肾炎的患者的肾活检标本在管状基底膜（BM）处显示含有补体 C3、免疫球蛋白（Ig）G 和 IgG4 的沉积物。目的是研究与非 AIP 患者相比，AIP 患者胰腺组织中补体和免疫球蛋白的沉积情况。
对 AIP 和酒精性慢性胰腺炎（ACP）患者的胰腺冷冻组织进行 C3c、IgG4 和 IgG 与 CK7、胰蛋白酶、胶原 IV、CD31 和 CD79a 的双重免疫荧光显微镜检查，以及 C1q、IgA 和 IgM 的免疫荧光显微镜检查。
在 AIP 患者中，补体 C3c、IgG4 和 IgG 沉积在胰腺和胆管的胶原 IV 阳性 BM 以及腺泡上。在少数 ACP 患者中，检测到较弱的 C3c 阳性 BM 沉积物，但不存在 IgG4 或 IgG 阳性 BM 沉积物。
C3c、IgG4 和 IgG 在小和中导管和胰腺腺泡的 BM 沉积是 AIP 的特征。这表明免疫复合物介导的导管和腺泡破坏在 AIP 的发病机制中起作用。
