新型多重实时 PCR 系统，利用 SNP 技术，用于同时诊断沙眼衣原体、解脲脲原体和人型支原体，并对非淋菌性尿道炎中沙眼衣原体和解脲脲原体的血清型进行基因分型。

Novel multiplex real-time PCR system using the SNP technology for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and genetic typing of serovars of C. trachomatis and U. parvum in NGU.

机构信息

The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.

出版信息

Mol Cell Probes. 2011 Feb;25(1):55-9. doi: 10.1016/j.mcp.2010.12.001. Epub 2010 Dec 15.

DOI:10.1016/j.mcp.2010.12.001

Abstract

To explore the possibilities of a novel multiplex real-time PCR system for rapid diagnosis, genetic typing of serovars and clinical application in NGU, we developed a multiplex real-time PCR system for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and molecular detection of serovars of C. trachomatis and U. parvum in NGU using the SNP technology and TaqMan-LNA probe. In 57 pathogen-positive clinical specimens, we identified the following C. trachomatis serovars: D (20.05%, 12/57), E (36.84%, 21/57), F (19.30%, 11/57), G (8.77%, 5/57), H (5.26%, 3/57), J (3.51%, 2/57), and K (5.26%, 3/57). In 115 pathogen-positive clinical specimens, we identified the following U. parvum serovars: 1 (0.87%, 2/115), 3 (55.65%, 64/115), 6 (20.87%, 24/115) and 14 (21.74%, 25/115). Our fast pathogen diagnosis and serotyping assay using real-time TaqMan-LNA PCR may improve our ability to study the pathogenesis and epidemiology of NGU.

摘要

为了探索新型多重实时 PCR 系统在快速诊断、血清型基因分型和 NGU 临床应用中的可能性，我们开发了一种用于同时诊断沙眼衣原体、解脲脲原体和人型支原体的多重实时 PCR 系统，并使用 SNP 技术和 TaqMan-LNA 探针进行分子检测。在 57 份病原体阳性的临床标本中，我们鉴定出以下沙眼衣原体血清型：D（20.05%，12/57）、E（36.84%，21/57）、F（19.30%，11/57）、G（8.77%，5/57）、H（5.26%，3/57）、J（3.51%，2/57）和 K（5.26%，3/57）。在 115 份病原体阳性的临床标本中，我们鉴定出以下解脲脲原体血清型：1（0.87%，2/115）、3（55.65%，64/115）、6（20.87%，24/115）和 14（21.74%，25/115）。我们使用实时 TaqMan-LNA PCR 进行的快速病原体诊断和血清分型检测可能会提高我们研究 NGU 发病机制和流行病学的能力。