Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
Nitric Oxide. 2011 Mar 15;24(2):84-90. doi: 10.1016/j.niox.2010.12.003. Epub 2010 Dec 15.
The nitric oxide (NO) donor sodium nitroprusside (SNP) is frequently used in plant science in vivo. The present in vitro study reveals its effects on the photosynthetic oxygen evolution and the chlorophyll fluorescence directly on isolated pea thylakoid membranes. It was found that even at very low amounts of SNP (chlorophyll/SNP molar ratio∼67:1), the SNP-donated NO stimulates with more than 50% the overall photosystem II electron transport rate and diminishes the evolution of molecular oxygen. It was also found that the target site for SNP-donated NO is the donor side of photosystem II. Compared with other NO-donors used in plant science, SNP seems to be the only one exhibiting stimulation of electron transport through photosystem II.
一氧化氮（NO）供体硝普钠（SNP）在植物科学中常被用于体内实验。本体外研究直接在分离的豌豆类囊体膜上揭示了其对光合氧气释放和叶绿素荧光的影响。结果发现，即使 SNP 的量非常低（叶绿素/SNP 摩尔比约为 67:1），SNP 供体的 NO 也能刺激超过 50%的整个光系统 II 电子传递速率，并减少分子氧的释放。还发现，SNP 供体的 NO 的靶标位点是光系统 II 的供体侧。与植物科学中使用的其他 NO 供体相比，SNP 似乎是唯一通过光系统 II 刺激电子传递的供体。
