Hagi H, Huldt G, Loftenius A, Schröder H
Department of Parasitology, Faculty of Medicine and Surgery, Somali National University, Mogadishu, Somalia.
Ann Trop Med Parasitol. 1990 Apr;84(2):171-9. doi: 10.1080/00034983.1990.11812451.
Antibody responses in schistosomiasis haematobium were studied in relation to age and infection intensity in Somalia. The area is highly endemic for Schistosoma haematobium but free of S. mansoni. Antibodies of the IgG class against particulate antigens of S. mansoni adult worms were investigated by immunofluorescence (gut and somatic associated antigens) and against soluble egg and adult worm antigens by ELISA. Total IgE levels were examined by Pharmacia IgE RIA, and specific IgE against soluble adult worm antigen by enzyme immunoassay. The IgG antibody response showed a characteristic pattern with highest reactivity against both gut associated and soluble egg antigens in the age group 10-14 years, when both prevalence and intensity of the infection were highest. Reactivity against somatic associated antigen was also high in this age group, but it increased slightly and remained at high level in the older ages. It is thought that such antigen is exposed mainly after the death of the parasite and that the antigenic stimulation may remain throughout most of the life of infected individuals. On the other hand, the IgG antibody reactivity against soluble adult worm antigen was low during childhood, but it increased significantly with age. It is suggested that repeated booster effects are needed for more potent response against these antigenic components. The finding of high levels of total IgE already in the youngest age groups, together with low specific IgE response, indicates that mainly other antigens are involved in the IgE production. The specific IgE response against soluble adult worm antigen was low but increased significantly with age.
在索马里，研究了埃及血吸虫病中的抗体反应与年龄和感染强度的关系。该地区埃及血吸虫病高度流行，但无曼氏血吸虫。通过免疫荧光法（针对肠道和体细胞相关抗原）研究了针对曼氏血吸虫成虫颗粒抗原的IgG类抗体，并通过酶联免疫吸附测定法研究了针对可溶性虫卵和成虫抗原的抗体。通过Pharmacia IgE放射免疫测定法检测总IgE水平，并通过酶免疫测定法检测针对可溶性成虫抗原的特异性IgE。IgG抗体反应呈现出一种特征模式，在10 - 14岁年龄组中，对肠道相关抗原和可溶性虫卵抗原的反应性最高，此时感染率和感染强度也最高。该年龄组对体细胞相关抗原的反应性也很高，但在较大年龄组中略有增加并保持在较高水平。据认为，此类抗原主要在寄生虫死亡后暴露，并且抗原刺激可能在受感染个体的大部分生命中持续存在。另一方面，针对可溶性成虫抗原的IgG抗体反应性在儿童期较低，但随年龄显著增加。有人提出，需要反复的增强作用才能对这些抗原成分产生更有效的反应。在最年幼的年龄组中就已发现高水平的总IgE，同时特异性IgE反应较低，这表明主要是其他抗原参与了IgE的产生。针对可溶性成虫抗原的特异性IgE反应较低，但随年龄显著增加。
