Department of Chemistry and Biochemistry, Oakwood University, 7000 Adventist Blvd., Huntsville, AL 35896, USA.
Bioelectrochemistry. 2011 Apr;81(1):4-9. doi: 10.1016/j.bioelechem.2010.11.001. Epub 2010 Nov 27.
Plant tissue has biologically closed electrical circuits and electric fields that regulate its physiology. The biologically closed electrochemical circuits in the leaves of Aloe vera were analyzed using the charge stimulation method with Ag/AgCl electrodes inserted along a leaf at 1-2 cm distance. The electrostimulation was provided with different timing and different voltages. Strong electrical anisotropy of the leaves was found. In the direction across the leaf the electrical circuits remained passive and linear, while along the leaf the response remained linear only at small voltages not exceeding 1 V. At higher potentials the circuits became strongly non-linear pointing to the opening of voltage gated ion channels in the plant tissues. Changing the polarity of electrodes located along conductive bundles led to a strong rectification effect and to different kinetics of capacitor discharge. Equivalent electrical circuit models of the leaf were proposed to explain the experimental data.
植物组织具有生物闭合的电路和电场，这些电路和电场调节着其生理学功能。本研究采用 Ag/AgCl 电极沿叶片以 1-2 厘米的间距插入的电荷刺激法分析了库拉索芦荟叶片中的生物闭合电化学电路。施加了不同时间和不同电压的电刺激。发现叶片具有很强的电各向异性。在垂直于叶片的方向上，电路保持被动和线性，而在沿着叶片的方向上，只有在不超过 1V 的小电压下，响应才保持线性。在更高的电位下，电路变得强烈非线性，表明植物组织中的电压门控离子通道打开。改变位于导电束沿线的电极的极性会导致强烈的整流效应和电容器放电的不同动力学。提出了叶片的等效电路模型来解释实验数据。
