Volkov Alexander G, Nyasani Eunice K, Tuckett Clayton, Scott Jessenia M, Jackson Mariah M Z, Greeman Esther A, Greenidge Ariane S, Cohen Devin O, Volkova Maia I, Shtessel Yuri B
Department of Chemistry, Oakwood University, 7000 Adventist Blvd., Huntsville, AL 35896, USA.
Department of Chemistry, Oakwood University, 7000 Adventist Blvd., Huntsville, AL 35896, USA.
Bioelectrochemistry. 2017 Feb;113:60-68. doi: 10.1016/j.bioelechem.2016.10.004. Epub 2016 Oct 12.
Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera. There is a difference in duration and amplitude of electrical potentials measured by electrodes inserted in a leaf and those attached to a leaf's surface. If the external reference electrode is located in the soil near the root, it changes the amplitude and duration of electrotonic potentials due to existence of additional resistance, capacitance, ion channels and ion pumps in the root. The information gained from this study can be used to elucidate extracellular and intercellular communication in the form of electrical signals within plants.
对植物进行电刺激可诱导植物运动、激活离子通道、促进离子运输、影响基因表达、激活酶系统、产生电信号、造成植物细胞损伤、加速伤口愈合并影响植物生长。在此，我们发现植物组织中的电网络具有电微分器。电响应的幅度沿叶片递减，且通过减小极化铂电极之间的距离而增大。将插入叶片的细胞间银/氯化银电极和附着在叶片表面的细胞外银/氯化银电极用于检测电紧张电位沿库拉索芦荟叶片的传播。插入叶片的电极与附着在叶片表面的电极所测得的电位在持续时间和幅度上存在差异。如果外部参比电极位于根部附近的土壤中，由于根部存在额外的电阻、电容、离子通道和离子泵，它会改变电紧张电位的幅度和持续时间。从这项研究中获得的信息可用于阐明植物体内以电信号形式存在的细胞外和细胞间通讯。
