Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Food Chem Toxicol. 2011 Apr;49(4):871-8. doi: 10.1016/j.fct.2010.12.010. Epub 2010 Dec 16.
Honey is reported to contain various compounds such as phenols, vitamins and antioxidants. The present study investigates the anticancer potential of Tualang honey (Agromas) (TH) in human breast (MCF-7 and MDA-MB-231) and cervical (HeLa) cancer cell lines; as well as in the normal breast epithelial cell line, MCF-10A. The cells were treated with increasing doses of TH (1-10%) for up to 72 h. Increase in lactate dehydrogenase (LDH) leakage from the cell membranes indicates that TH is cytotoxic to all three cancer cells with effective concentrations (EC(50)) of 2.4-2.8%. TH is however, not cytotoxic to the MCF-10A cells. Reactivity with annexin V fluorescence antibody and propidium iodide as analysed by flow cytometry and fluorescence microscopy shows that apoptosis occurred in these cancer cells. TH also reduced the mitochondrial membrane potential (Δψ(m)) in the cancer cell lines after 24h of treatment. The activation of caspase-3/7 and -9 was observed in all TH-treated cancer cells indicating the involvement of mitochondrial apoptotic pathway. This study shows that TH has significant anticancer activity against human breast and cervical cancer cell lines.
蜂蜜据称含有多种化合物，如酚类、维生素和抗氧化剂。本研究调查了图蓝本蜂蜜（Agromas）（TH）对人乳腺癌（MCF-7 和 MDA-MB-231）和宫颈癌（HeLa）细胞系以及正常乳腺上皮细胞系 MCF-10A 的抗癌潜力。用递增剂量的 TH（1-10%）处理细胞长达 72 小时。从细胞膜漏出的乳酸脱氢酶（LDH）增加表明 TH 对所有三种癌细胞均具有细胞毒性，有效浓度（EC（50））为 2.4-2.8%。然而，TH 对 MCF-10A 细胞没有细胞毒性。通过流式细胞术和荧光显微镜分析与膜联蛋白 V 荧光抗体和碘化丙啶的反应表明，凋亡发生在这些癌细胞中。TH 在处理 24 小时后还降低了癌细胞系中的线粒体膜电位（Δψ（m））。在所有 TH 处理的癌细胞中均观察到 caspase-3/7 和 -9 的激活，表明涉及线粒体凋亡途径。这项研究表明，TH 对人乳腺癌和宫颈癌细胞系具有显著的抗癌活性。
