Department of Food Science, Quality & Technology, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Copenhagen, Frederiksberg C, Denmark.
Anal Chim Acta. 2011 Jan 10;683(2):170-7. doi: 10.1016/j.aca.2010.10.014. Epub 2010 Oct 30.
In this work, the time-dependent conjugation process between a thiolated molecule (with anti-parkinsonian properties) and gold nanoparticles has been monitored and studied by the combined use of fast acquisition Ultra Violet-Visible (UV-Vis) spectra and the ability of Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) technique. From the highly informative kinetic profiles obtained it was possible to extract quantitative and qualitative information of the conjugation process which includes i) time-dependent concentration profiles and pure spectra of species involved on conjugation process, ii) estimation of molecule concentration necessary for the completeness of the conjugation reaction, iii) molecule footprint and iv) free energy of molecule adsorption.
在这项工作中，通过结合使用快速采集的紫外-可见（UV-Vis）光谱和多变量曲线分辨-交替最小二乘法（MCR-ALS）技术，监测并研究了巯基分子（具有抗帕金森病特性）与金纳米粒子之间的时变共轭过程。从获得的高度信息丰富的动力学曲线中，可以提取出共轭过程的定量和定性信息，包括：i）时变浓度曲线和参与共轭过程的物种的纯光谱，ii）完成共轭反应所需的分子浓度估计，iii）分子足迹和 iv）分子吸附自由能。
