Liu Xiong, Atwater Mark, Wang Jinhai, Huo Qun
Nanoscience and Nanotechnology Center and Chemistry Department, University of Central Florida, 12424 Research Pkwy, Orlando, FL 32826, USA.
Colloids Surf B Biointerfaces. 2007 Jul 1;58(1):3-7. doi: 10.1016/j.colsurfb.2006.08.005. Epub 2006 Aug 17.
Extinction coefficients of gold nanoparticles with core size ranging from approximately 4 to 40 nm were determined by high resolution transmission electron microscopy analysis and UV-vis absorption spectroscopic measurement. Three different types of gold nanoparticles were prepared and studied: citrate-stabilized nanoparticles in five different sizes; oleylamide-protected gold nanoparticles with a core diameter of 8 nm, and a decanethiol-protected nanoparticle with a diameter of around 4 nm. A linear relationship between the logarithms of extinction coefficients and core diameters of gold particles was found independent of the capping ligands on the particle surface and the solvents used to dissolve the nanoparticles. This linear relation may be used as a calibration curve to determine the concentration or average size of an unknown nanoparticle or nanoparticle-biomolecule conjugate sample.
通过高分辨率透射电子显微镜分析和紫外-可见吸收光谱测量，测定了核尺寸范围约为4至40纳米的金纳米颗粒的消光系数。制备并研究了三种不同类型的金纳米颗粒：五种不同尺寸的柠檬酸盐稳定的纳米颗粒；核直径为8纳米的油酰胺保护的金纳米颗粒，以及直径约为4纳米的癸硫醇保护的纳米颗粒。发现金颗粒的消光系数对数与核直径之间存在线性关系，该关系与颗粒表面的封端配体以及用于溶解纳米颗粒的溶剂无关。这种线性关系可作为校准曲线，用于确定未知纳米颗粒或纳米颗粒-生物分子共轭物样品的浓度或平均尺寸。
