Cotariu D, Evans S, Zaidman J L, Marcus O
Department of Biochemical Pathology, Assaf Harofeh Medical Center, Sackler School of Medicine, Zerifin, Israel.
Biochem Pharmacol. 1990 Aug 1;40(3):589-93. doi: 10.1016/0006-2952(90)90560-8.
Changes in reduced glutathione (GSH) and pyridine nucleotide phosphate levels as well as in the activities of the glutathione peroxidase-reductase system and glucose-6-phosphate dehydrogenase have been studied in rats after a single i.p. administration of various doses of valproic acid (VPA). GSH level decreased in a dose-dependent relation. At the end of 180 min GSH levels either returned to control limits (lower doses) or showed a tendency to normalize (higher doses). GSH loss was paralleled by the reduction in glutathione reductase activity. A significant NADPH reduction was also seen after animal exposure to high VPA doses. At the end of 180 min a maximal NADPH decrease was reached. The activities of both glutathione peroxidase and glucose-6-phosphate dehydrogenase were suppressed irrespective of whether animals were given low or high VPA doses.
在大鼠腹腔注射单次不同剂量丙戊酸（VPA）后，研究了还原型谷胱甘肽（GSH）和吡啶核苷酸磷酸水平的变化，以及谷胱甘肽过氧化物酶-还原酶系统和葡萄糖-6-磷酸脱氢酶的活性。GSH水平呈剂量依赖性降低。在180分钟结束时，GSH水平要么恢复到对照范围（较低剂量），要么呈现出趋于正常化的趋势（较高剂量）。GSH的减少与谷胱甘肽还原酶活性的降低平行。在动物暴露于高剂量VPA后，还观察到显著的NADPH减少。在180分钟结束时达到最大的NADPH降低。无论给动物注射低剂量还是高剂量的VPA，谷胱甘肽过氧化物酶和葡萄糖-6-磷酸脱氢酶的活性均受到抑制。
