Centre de Génétique Moléculaire, CNRS FRE 3144, Avenue de la terrasse, Gif-sur-Yvette F-91190, France.
Mitochondrion. 2011 May;11(3):391-6. doi: 10.1016/j.mito.2010.12.002. Epub 2010 Dec 16.
Starting from a transcriptome based study of the spatio-temporal expression of yeast genes encoding mitochondrial proteins of unknown function, we have identified the gene BCA1 (YLR077W). A FISH analysis showed that the BCA1 mRNA co-localized with the mitochondrial network. Cellular fractionation revealed that Bca1 is bound to the mitochondrial inner-membrane and protrudes into the inter-membrane space. We show that Bca1 controls an early step in complex III assembly and that the supra-molecular organization of Bca1 is dependent upon the assembly level of complex III. Thus, Bca1 is a novel assembly factor for the respiratory complex III.
从基于转录组的酵母基因时空表达研究开始，这些基因编码线粒体未知功能的蛋白质，我们鉴定了基因 BCA1（YLR077W）。FISH 分析显示，BCA1mRNA 与线粒体网络共定位。细胞分级分离显示 Bca1 与线粒体内膜结合并突出到膜间隙中。我们表明 Bca1 控制复合物 III 组装的早期步骤，并且 Bca1 的超分子组织取决于复合物 III 的组装水平。因此，Bca1 是呼吸复合物 III 的一种新型组装因子。
