Khatri Yogan, Hannemann Frank, Ewen Kerstin M, Pistorius Dominik, Perlova Olena, Kagawa Norio, Brachmann Alexander O, Müller Rolf, Bernhardt Rita
Department of Biochemistry, Saarland University, Campus B2.2, 66123 Saarbrücken, Germany.
Chem Biol. 2010 Dec 22;17(12):1295-305. doi: 10.1016/j.chembiol.2010.10.010.
The first systematic study of the complete cytochrome P450 complement (CYPome) of Sorangium cellulosum So ce56, which is a producer of important secondary metabolites and has the largest bacterial genome sequenced to date, is presented. We describe the bioinformatic analysis of the So ce56 cytochrome P450 complement consisting of 21 putative P450 genes. Because fatty acids play a pivotal role during the complex life cycle of myxobacteria, we focused our studies on the characterization of fatty acid hydroxylases. Three novel potential fatty acid hydroxylases (CYP109D1, CYP264A1, and CYP266A1) were used for detailed characterization. One of them, CYP109D1 was able to perform subterminal hydroxylation of saturated fatty acids with the support of two autologous and one heterologous electron transfer system(s). The kinetic parameters for the product hydroxylation were derived.
对纤维素堆囊菌So ce56完整的细胞色素P450基因库（CYPome）进行了首次系统研究，纤维素堆囊菌So ce56是重要次生代谢产物的生产者，其细菌基因组是迄今为止已测序的最大细菌基因组。我们描述了由21个假定的P450基因组成的So ce56细胞色素P450基因库的生物信息学分析。由于脂肪酸在粘细菌复杂的生命周期中起关键作用，我们将研究重点放在脂肪酸羟化酶的表征上。对三种新型潜在脂肪酸羟化酶（CYP109D1、CYP264A1和CYP266A1）进行了详细表征。其中一种，CYP109D1能够在两个自体和一个异源电子转移系统的支持下对饱和脂肪酸进行亚末端羟基化。得出了产物羟基化的动力学参数。
